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ProteomicsLinc-ADAIN Pulldown assay in Human Adipocytes.
Description: a biotinylated pulldown assay of linc-ADAIN, lincRNA vs Antisense Linc-ADAIN in primary human ASC adipocytes coupled to mass spectrometry (MS).
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD051388 | Pride
Pulldown assay Linc-rna protein interaction Adipocytes Lincrna Linc-adain 
ProteomicsKinase Inhibitor Pulldown Assay (KiP) for Clinical Proteomics
Protein kinases are frequently dysregulated and/or mutated in cancer and represent essential targets for therapy. Accurate quantification is essential, but current approaches are inadequate. For breast cancer treatment for example, the identification and quantification of the protein kinase ERBB2 is...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044655 | Pride
Human Breast Cancer Kinase 
ProteomicsKinase Inhibitor Pulldown Assay (KiP) for Clinical Proteomics (SureQuant)
Protein kinases are frequently dysregulated and/or mutated in cancer and represent essential targets for therapy. Accurate quantification is essential, but current approaches are inadequate. For breast cancer treatment for example, the identification and quantification of the protein kinase ERBB2 is...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD046169 | Pride
Breast cancer Is-prm Surequant Kip 
TranscriptomicsGenome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition
Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete. In the RNAcompete assay, purified GST-tagged BRAT is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agil...
ORGANISM(S): synthetic construct 
2015-03-30 | E-GEOD-60498 | biostudies-arrayexpress
TranscriptomicsThe crystal structure of the NHL domain in complex with RNA reveals the molecular basis of Drosophila Brain tumor-mediated gene regulation
Here, we analyze the RNA-binding preferences of several NHL containing RNA-binding proteins (BRAT, NCL-1, TRIM71, WECH, LIN-41, TRIM56, and MEI-P26) using RNAcompete. In the RNAcompete assay, purified GST-tagged RNA-binding protein is incubated with an excess of RNA pool and bound RNA from individua...
ORGANISM(S): synthetic construct 
2015-09-14 | E-GEOD-73000 | biostudies-arrayexpress
TranscriptomicsConserved functional antagonism between CELF and MBNL proteins regulates stem cell-specific alternative splicing and regeneration in planarians
Here, we analyze the RNA-binding preferences of the planarian smed-mbnl-like-2, smed-mbnl-1 (isoform X1), smed-bruli, smed-mbnl-1 (isoform Xins), and smed-mbnl-like-1 protein using RNAcompete. In the RNAcompete assay, a purified GST-tagged protein is incubated with an excess of RNA pool and bound RN...
ORGANISM(S): synthetic construct 
2016-08-01 | E-GEOD-75554 | biostudies-arrayexpress
GenomicsGenome wide hydroxymethylation tested using the HELP-GT assay shows redistribution in cancer
Background: 5-hydroxymethylcytosine (5-hmC) is a recently discovered epigenetic modification that is altered in cancers. Genome wide assays for 5-hmC determination are needed as many of the techniques commonly used to assay 5-methylcytosine (5-mC), including conventional methyl-sensitive restriction...
ORGANISM(S): Homo sapiens 
2013-07-30 | E-GEOD-42723 | biostudies-arrayexpress
GenomicsIdentification of DAMER-interacting RNAs by RNA-RNA pulldown assay and sequencing [RNA-Seq]
Identification of DAMER-interacting RNAs by RNA-RNA pulldown assay and sequencing [RNA-Seq]
PRJNA1336083 | ENA
UnknownKinase inhibitor pulldown assay (KiP) for clinical proteomics.
Not available
S-EPMC10790396 | biostudies-literature
GenomicsPulldown DNA methylation study v2
Pilot study to set up sequencing protocols for targeted pulldown methylation profiling
EGAS00001000979 | EGA
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