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ProteomicsProtrusion vs Cell-body pulsed-SILAC proteomics
In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. Apart from proteins, several mRNAs are known to be localised to cell protrusions but to what extent RNA localisation and local translation contributes toward front-back assymetry is unknown...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2015-10-09 | PXD002649 | Pride
Pulsed-silac  local translation  protrusion 
ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsPulsed-SILAC analysis of protein translation rate changes upon protrusion formation
To assess the impact of protrusion formation on protein synthesis rates on a global scale, we carried out a pulsed-SILAC (pSILAC) analysis in MDA-MB231 cells grown with or without protrusions for 1, 2, 4, and 8 hrs. Briefly, light SILAC labelled cells were seeded on top of 2x 3 micron transwell filt...
ORGANISM(S): Homo sapiens (Human) 
2020-10-20 | PXD021203 | Pride
Pulsed-silac  psilac Mda-mb231  protrusions translation  orbitrap 
ProteomicsAge-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
Collagen-rich tissues have poor reparative capacity that is further impaired with age, predisposing to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, skin and plasma of mice acro...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-23 | PXD023180 | Pride
Collagen turnover  pulse silac Mouse 
ProteomicsPulsed SILAC study of cell lines following PPIA knockdown
Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spec...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-16 | MSV000093125 | MassIVE
ProteomicsPulsed SILAC quantitative proteomics profiling to understand the protective role of ginsentide TP1 against hypoxia.
This project used a Pulsed SILAC (pSILAC) proteomics approach to pulsed/chase the incorporation of stable isotope-labeled lysine (13C6 and 15N2 L-lysine) and arginine (13C6 L-arginine) into newly synthesized proteins and profiled de novo protein synthesis in endothelial cells (HUVEC-CS) subjected to...
ORGANISM(S): Homo Sapiens (human) 
2024-02-08 | PXD039938 | JPOST Repository
ProteomicsEnrichment and quantitation of newly secreted proteins under serum condition by combining BONCAT and pulsed SILAC
Despite the increasing interest of secretome involved in paracrine/autocrine mechanisms, the majority of the mass spectrometric cell secretome studies have been performed using serum-free medium (SFM), due to low-abundance of secreted proteins and high-abundance of contaminating fetal bovine serum (...
ORGANISM(S): Homo sapiens (Human) 
2019-03-13 | PXD007529 | Pride
Boncat Serum Fbs Secretome Pulsed silac Lc-ms/ms 
ProteomicsProtein and peptide turnover measurements using pulsed SILAC-TMT approach
We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved in...
ORGANISM(S): Homo sapiens (Human) 
2018-02-09 | PXD008579 | Pride
Degradation rates Protein turnover Proteoforms Posttranslational cleavage Synthesis rates Tmt Splice variants Pulsed silac Posttranslational modification 
UnknownNew RoxS sRNA Targets Identified in Bacillus subtilis by Pulsed SILAC.
Not available
S-EPMC10433868 | biostudies-literature
ProteomicsSpatial SILAC Regorafenib Treated Bottom-up Proteomic Analysis
Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model sys...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD038958 | Pride
Spatial silac Regorafenib Spheroids 
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