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ProteomicsNovel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation
Monitoring protein synthesis is essential to our understanding of gene expression regulation, as protein abundance is thought to be predominantly controlled at the level of translation. Mass-spectrometric and RNA sequencing methods have been recently developed for investigating mRNA translation at a...
ORGANISM(S): Homo sapiens (Human) 
2019-02-18 | PXD012727 | Pride
Translatome Ribosome Punch-p 
TranscriptomicsComplementary Post Transcriptional Regulatory Information is Detected by PUNCH-P and Ribosome Profiling
Two novel approaches were recently suggested for genome-wide identification of protein aspects synthesized at a given time. Ribo-Seq is based on sequencing all the ribosome protected mRNA fragments in a cell, while PUNCH-P is based on mass-spectrometric analysis of only newly synthesized proteins. H...
ORGANISM(S): Homo sapiens 
2016-09-28 | GSE73565 | GEO
ProteomicsMeasuring C2C12 Myotube Protein Expression Changes in Inflammation using Punch-P
Selective identification of newly synthesized proteins provides insight on biological processes that depend on the continued synthesis of specific proteins to maintain homeostasis as well as those that are up- or down-regulated in response to changing needs. We used puromycin-associated nascent chai...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-28 | PXD003144 | Pride
Lps Mouse Inflammatio Interferon gamma 5600 tripletof Muscle Myotubes 
UnknownPUNCH-P for global translatome profiling: Methodology, insights and comparison to other techniques.
Not available
S-EPMC4718054 | biostudies-literature
UnknownComplementary Post Transcriptional Regulatory Information is Detected by PUNCH-P and Ribosome Profiling.
Not available
S-EPMC4761937 | biostudies-literature
GenomicsHomo sapiens
Complementary Post Transcriptional Regulatory Information is Detected by PUNCH-P and Ribosome Profiling
PRJNA297338 | ENA
TranscriptomicsTranscriptomic comparison of young and aged choroid at the bulk and single-cell levels (bulk)
Purpose: The goals of this study were to identify cell specific expression patterns from infant and aged choroid, with a particular emphasis on how endothelial gene expression changes with age. Methods: Independent libraries were prepared for infant and adult choroidal cells from seventeen human don...
ORGANISM(S): Homo sapiens 
2020-04-23 | GSE149099 | GEO
TranscriptomicsEpicardial Placement of Human Placental Membrane Protects from Heart Injury in a Swine Model of Myocardial Infarction
Introduction: Cardiac ischemic reperfusion injury (IRI) is paradoxically instigated by re-establishing blood-flow to ischemic myocardium typically from a myocardial infarction (MI). Although revascularization following MI remains the standard of care, effective strategies remain limited to prevent o...
ORGANISM(S): Sus scrofa domesticus 
2023-05-30 | GSE228096 | GEO
UnknownDevelopment of a tool to assess HIV prevention readiness of adolescent girls and young women in HPTN 082 study.
Not available
S-EPMC9956790 | biostudies-literature
TranscriptomicsGene expression analysis of chicken p. major muscle using the NanoString nCounter technology
Tissue samples were harvested with the Keyes biopsy punch (8 mm; manufactured by HNM) from the caudal edge of the right superficial pectoral muscle perpendicular to myofibers and to the keel bone. Total RNA was isolated from p. major samples using mirVana™ miRNA Isolation Kit. Quality checks were pe...
ORGANISM(S): Gallus gallus 
2021-01-11 | GSE162634 | GEO
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