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ProteomicsMS to detect peptides of human HCT116 cell lysate immunoprecipitated by anti-miPEP31
We used anti-miPEP31 to immnoprecipitated the interacting protein or peptides. Then segregate ingredients ≤ 10 kD and > 3 kD. All mass spectrometric experiments were performed on a QE-Plus mass spectrometer connected to an Easy-nLC 2000 via an Easy Spray (ThermoFisher Scientific).
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD029131 | Pride
Human Hct116 Ip-lc-ms Peptides 
ProteomicsProteome dynamics during transition from exponential to stationary phase in yeast
Glycolysis is the central pathway for sugar metabolism in most living organisms. The single celled eukaryote yeast is a widely used model organism for higher eukaryotes in which the regulation of glycolysis is widely studied. S. cerevisiae is one of the few eukaryotic organisms that can efficiently ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-04-15 | PXD031412 | Pride
Growth Yeast Tmt quantification Orbitrap qe plus Glycolysis 
ProteomicsOptimizing chromatographic conditions for orbitrap-based MS platforms
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
ORGANISM(S): Homo sapiens (Human) 
2019-03-04 | PXD009875 | Pride
Reproducibility Data-dependent acquisition Chromatography Q exactive Fusion lumos Human Pearson product-moment correlation coefficient Distributed normalized spectral abundance factor Orbitrap Quantitative proteomics Multidimensional protein identification technology 
ProteomicsIdentification of peptides from E-Jiao
E-Jiao is a traditional Chinese medicine. In this project, we used Q Exactive Orbitrap Plus to identify the peptides from E-Jiao. We also used the QE Plus to investigate the E-Jiao peptides in rat blood.
ORGANISM(S): Equus caballus (Horse) 
2024-07-03 | PXD052456 | Pride
E-jiao  q exactive orbitrap plus Donkey hide gelatin 
ProteomicsProteomic analysis of alcohol and LPS-treated primary mouse microglia
Microglia, the resident immune cells of the brain, can exhibit a broad range of activation phenotypes, many of which have been implicated in several diseases and disorders of the central nervous system including alcohol use disorders and disorders. By utilizing a method optimized for sensitive and ...
ORGANISM(S): Mus musculus (Mouse) 
2020-04-07 | PXD014466 | Pride
Lps Neuroinflammation Ethanol Alcohol Microglia Glia 
ProteomicsPutative methylomirabilis lanthanidiphila surface layer protein
Gambelli et al., 2021, investigate methylomirabilis bacteria, which perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway to produce carbon dioxide and dinitrogen gas. These bacteria possess an unusual polygonal cell shape with sharp ridges that run along the...
ORGANISM(S): Candidatus Methylomirabilis sp. lanth 
2022-01-06 | PXD029319 | Pride
Qe plus Methylomirabilis lanthanidiphila Surface layer 
MetabolomicsGenetic Ablation of Sfxn5 Induces Mitochondrial Dysfunction and Precipitates Lethal Metabolic Crisis in Mice
Mitochondrial disorders frequently manifest with life-threatening hepatic metabolic crises. Using a global Sfxn5-knockout mouse model, we investigated the systemic consequences of disrupting this mitochondrial inner-membrane transporter through integrated biochemical, metabolomic, histological, and ...
2026-05-30 | MTBLS14628 | MetaboLights
ProteomicsEngineered yeast strains expressing human glycolytic enzymes
Ewout Knibbe et al.,(Pascale Daran-Lapujade Lab, TU Delft) report on engineered yeast strains to express the human glycolysis pathway instead of the native pathway enzymes. The goal of the project is to investigate the conservation of these functions between species. The engineered yeast strains wer...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-12-22 | PXD025349 | Pride
Qe plus Yeast Tmt experiment Glycolysis 
ProteomicsProteomic analysis of sulfur-respiring haloarchaea from hypersaline lakes
Dimitry Y. Sorokin et al., (2021, Russian Academy of Sciences, Russia and Delft University of Technology, The Netherlands) describe the isolation and physiological and genomic properties of a fifth functional group of sulfur-respiring haloarchaea enriched from hypersaline lake sediments with CO as t...
ORGANISM(S): Archaea 
2022-12-25 | PXD028241 | Pride
Shotgun discovery proteomics Haloarchaea Qe plus 
ProteomicsH3.3K27M HLA-A2 is not an effective immunotherapy target for diffuse midline glioma
The study investigated presentation of HLA A2 restricted H3.3K27M neopeptide using immunopeptidomics followed by DDA and/or targeted multiple monitoring reaction (MRM).
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD038371 | Pride
Hla Car t cells H3.3 k27m Dipg Immunopeptidomics 
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