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ProteomicsQuantitative Top-Down Proteomics Analysis of Age-related Changes in Murine Hearts
Label-free quantitative top-down proteomics was performed on mitochondrial lysate isolated from the hearts of 3 young (4-month-old) and 3 old (24-25-month-old) mice.
ORGANISM(S): Mus musculus (Mouse) 
2024-10-17 | PXD051505 | Pride
Top-down Quantitative Aging 
ProteomicsCysteine-directed isobaric labeling combined with GeLC-FAIMS MS for quantitative top-down proteomics
The quantification of proteoforms, i.e., all molecular forms in which proteins can be present, by top-down proteomics provides essential insights into biological processes at the molecular level. Isobaric labeling-based quantification strategies are suitable for multi-dimensional separation strategi...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2025-05-07 | PXD055510 | Pride
Top-down proteomics  tmt labeling  quantitative proteomics  faims  gelc  peppi 
ProteomicsSarcomeric Proteoform Landscape in Ischemic Cardiomyopathy Defined by Top-down Proteomics
In ischemic cardiomyopathy (ICM), left ventricular systolic dysfunction leads to reduced blood flow and oxygen supply to the heart. Alterations in sarcomeric protein function and expression play prominent roles in the onset and progression of cardiomyopathies; however, the molecular mechanisms under...
ORGANISM(S): Homo sapiens (Human) 
2023-02-20 | PXD038066 | Pride
Ischemic cardiomyopathy Top-down proteomics Sarcomere Z-disk proteins Myofilament proteins Quantitative proteomics Human heart proteomics 
ProteomicsUniversal Solid-Phase Protein Preparation (USP3) for bottom-up and top-down proteomics
Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical to the success of quantitative workflows. Here we present a universal, solid-phase protein preparation (USP3) method which is rapid, robust and scalable, facilitating high-throughput protein sample preparation...
ORGANISM(S): Homo sapiens (Human) 
2019-06-05 | PXD011169 | Pride
Top-down proteomics Universal solid-phase protein preparation Bottom-up proteomics Intact mass spectrometry 
ProteomicsTop Down Histone H4 - SUM159 Butyrate
Proteins physiologically exist and function as ‘proteoforms’ that arise from one gene but acquire additional function by further variation such as post-translational modification (PTM). When multiple PTMs coexist on single protein molecules top down proteomics becomes the only feasible method; howev...
ORGANISM(S): Homo sapiens (Human) 
2019-11-22 | PXD013766 | Pride
Top-down Histone h4 
ProteomicsQuantitative top-down proteomics reveals significant differences in histone proteoforms between metastatic and nonmetastatic colorectal cancer cells
As one of the leading causes of cancer deaths worldwide, colorectal cancer (CRC) development is closely associated with the accumulation of both genetic and epigenetic alterations. Many efforts have been made to investigate the role of epigenetic modifications in CRC metastasis. In this work, we pre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD060160 | Pride
Top-down proteomics Post-translational modifications Capillary electrophoresis-mass spectrometry Colorectal cancer metastasis Histone proteoforms 
ProteomicsSpatially-Resolved Top-Down Proteomics of Zebrafish Brain Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry
Understanding region-specific proteoform profiles in the brain is crucial for deciphering neural function and identifying potential therapeutic targets. Here, we present a comprehensive label-free quantitative top-down proteomics (TDP) analysis of distinct zebrafish brain regions using capillary zon...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD070925 | Pride
Proteoform Top-down proteomics Zebrafish brainregions 
ProteomicsQuantitative Top-Down Proteomics Revealed Staurosporine-induced Proteoform-level Changes in Cancer Cells
We conducted a quantitative top-down proteomics analysis on HeLa cells treated with staurosporine to investigate differential expression of proteoforms. Furthermore, we assessed alterations in phosphorylation patterns for intact phosphoproteoforms
ORGANISM(S): Homo sapiens (Human) 
2025-01-31 | PXD048148 | Pride
Proteomics Hela Phosphorylation Quantitative Top-down Staurosporine 
ProteomicsCZE-MS/MS-based quantitative top-down proteomics reveals significant proteoform differences between healthy and Alzheimer's disease human brains
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive decline and pathological protein aggregation. Comprehensive protein characterization of human brains of AD patients and comparison with healthy samples is critical for pursuing a better understanding of the molecular...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD065459 | Pride
Top-down proteomics Human brain Proteoform Tau phosphorylation. Cze-ms/ms Alzheimer's disease 
ProteomicsAutomated capillary isoelectric focusing-tandem mass spectrometry for top-down proteomics
Mass spectrometry (MS)-based top-down proteomics (TDP) delineates proteoforms in cells and requires high-resolution proteoform separation. Herein, for the first time, we employed an automated capillary isoelectric focusing-tandem MS (cIEF-MS/MS) system for large-scale TDP of complex proteomes. Singl...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Escherichia coli 
2020-12-08 | PXD020342 | Pride
Endogenous hormone proteoforms/gel-free 2d-page/multi-level proteomics/ sexual dimorphism/zebrafish brain 
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