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UnknownCross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification.
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S-EPMC4813705 | biostudies-literature
ProteomicsHuman non-small cell lung cancer cell line NCI-H661 DPPA2-4 rapid immunoprecipitation mass spectrometry (RIME, LC-MSMS)
To investigate DPPA2/4 bound proteins on chromatin we performed rapid immunoprecipitation mass spectrometry (RIME) following double crosslinking of chromatin in NCI-H661 non-small cell lung cancer cells clonal cell lines (Wild Type clones c51 & c52, DPPA2 knockout clones c41 & c42, DPPA4 knockout cl...
ORGANISM(S): Homo sapiens (Human) 
2026-03-31 | PXD070627 | Pride
Rime Dppa4 Non small cell lung cancer Ncih661 Dppa2 Human 
ProteomicsComplementary omics strategies to dissect p53 signaling networks under nutrient stress
Signaling trough cytoplasmic or nuclear action of p53 is a major response mechanism to cellular stresses. While p53 protein levels have been shown to increase upon nutrient stresses such as starvation, the exact signaling cascade in this context remains elusive. Here, we show in a human hepatoma cel...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD028341 | Pride
Response to starvation Human hepatoma cell line Rapid immunoprecipitation ms P53 
ProteomicsIdentification of Endos interacting proteins in Drosophila embryos by immunoprecipitation and LC-MS-MS/MS analysis
The transition from oocyte to embryo requires translation of maternally provided transcripts that in Drosophila is activated by Pan Gu kinase to release a rapid succession of 13 mitotic cycles. Mitotic entry is promoted by several protein kinases that include Greatwall/Mastl, whose endosulfine subst...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2024-08-09 | PXD036613 | Pride
Endos Syncytial embryo Lc-ms-ms/ms Immunoprecipitation Drosophila 
TranscriptomicsStably-paused genes revealed through inhibition of transcription initiation by the TFIIH inhibitor Triptolide
Transcription by RNA Polymerase II (Pol II) in metazoan is regulated in several steps, including preinitiation complex (PIC) formation, initiation, Pol II escape, productive elongation, cotranscriptional RNA-processing and termination. Genome-wide studies have demonstrated that the phenomenon of ...
ORGANISM(S): Homo sapiens 
2015-01-05 | E-GEOD-63314 | biostudies-arrayexpress
ProteomicsAndrogen receptor RIME using LNCaP cells
Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) was conducted to examine interactome of androgen receptor (AR) in LNCaP cells.
ORGANISM(S): Homo Sapiens (human) 
2021-09-10 | PXD024030 | JPOST Repository
TranscriptomicsEvolution of human-specific microRNA miR-941 [Ago2_IP]
miRNA-mediated gene expression silencing has previously been shown to be important for a variety of physiological and pathological processes. Here, we have explored the role of one bona fide human-specific miRNA (miR-941) in evolution of the human-specific expression and function. Using combination ...
ORGANISM(S): Homo sapiens 
2012-12-20 | E-GEOD-39227 | biostudies-arrayexpress
ProteomicsA PEROXO-Tag enables rapid isolation of peroxisomes from human cells
Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than one hour to complete and can be laborious to implement. To address this, we have now extended our prior work on rapid organell...
ORGANISM(S): Homo sapiens (Human) 
2020-05-20 | PXD018918 | Pride
Human hek-293t Peroxisome 
ProteomicsA PEROXO-Tag enables rapid isolation of peroxisomes from human cells
Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than one hour to complete and can be laborious to implement. To address this, we have now extended our prior work on rapid organell...
ORGANISM(S): Homo sapiens (Human) 
2020-05-20 | PXD018880 | Pride
Human hek-293t Peroxisome Peroxo-ip 
ProteomicsIdentification of BNC2 interactors in human LX2 cells using RIME
The goal of this study was to use Rapid Immunoprecipitation Mass spectrometry of Endogenous protein (RIME) assays (Mohammed et al., 2016) to identify transcriptional regulatory partners of the transcription factor BNC2 in LX2 cells. Protein complexes were immune-precipitated with an anti-BNC2 antibo...
ORGANISM(S): Homo sapiens (Human) 
2022-08-05 | PXD003624 | Pride
Rapid immunoprecipitation mass spectrometry of endogenous proteins (rime)  human  lx2  hepatic stellate cells  bnc2 
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