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ProteomicsQuantitative cross-linking/mass spectrometry reveals subtle protein conformational changes
We have developed quantitative cross-linking/mass spectrometry (QCLMS) to interrogate conformational rearrangements of proteins in solution. Our workflow was tested using a structurally well-described reference system, the human complement protein C3 and its activated cleavage product C3b. We found ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-06-16 | MSV000079827 | MassIVE
GenomicsEic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly
CENP-A chromatin the foundation for kinetochore assembly in all eukaryotes. CENP-A is incorporated outside of S phase and depends on its chaperone HJURPScm3, and Mis18 in vertebrates and fission yeast. The recruitment of Mis18 and HJURPScm3 to centromeres is cell cycle regulated. Vertebrate Mis18 a...
ORGANISM(S): Schizosaccharomyces pombe 
2014-05-19 | E-GEOD-54685 | biostudies-arrayexpress
ProteomicsA folded conformation of MukBEF and Cohesin
Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the und...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-03-01 | PXD012377 | Pride
Condensin Structural maintenance of chromosomes Loop extrusion Smc proteins Crosslinking Mukbef Cohesin Dna translocation Kleisin 
ProteomicsCross-Linking/mass spectrometry (CLMS) analysis of RNA polymerase II
The mass spectrometry data of a previous publication (Chen et.al. 2009 doi:10.1038/emboj.2009.401) were reprocesed and is used for method developing, teaching and training purpose.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-07-20 | PXD010481 | Pride
Cross-linking/mass spectrometry Mult-iprotein complex 
ProteomicsA folded conformation of MukBEF and Cohesin
PleasStructural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but th...
ORGANISM(S): Escherichia coli 
2019-03-01 | PXD012370 | Pride
Condensin Structural maintenance of chromosomes Loop extrusion Smc proteins Crosslinking Mukbef Cohesin Dna translocation Kleisin 
ProteomicsCross-linking/mass spectrometry analysis of the AOC complex
As a part of a study on how kinetochors are assembled at the centromeres of the chromosomes, cross-linking/mass spectrometry has been applied to investigate interactions between Okp1/ Ame1 heterodimer, which is part of the COMA complex, and CENP-A from Saccharomyces cerevisiae.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-30 | PXD009648 | Pride
Protein interaction Cenp-a Okp1 Centromere Cross-linking/mass spectrometry Ame1 
ProteomicsCryo-EM structures of dynactin’s shoulder and pointed end show their complex architecture and reveal conserved cargo adaptor binding sites
Dynactin is a 1.2 MDa complex that activates the molecular motor, dynein, for ultra-processive transport along microtubules. In order to do this it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible...
ORGANISM(S): Homo sapiens (Human) 
2021-03-19 | PXD020084 | Pride
Dynactin Crosslinking ms 
ProteomicsUltraviolet Photodissociation of tryptic peptide backbones at 213 nm
We analyzed the backbone fragmentation behavior of tryptic peptides of a four protein mixture and of E. coli lysate subjected to Ultraviolet Photodissociation (UVPD) at 213 nm on a commercially available UVPD-equipped tribrid mass spectrometer. We obtained 15,178 high-confidence peptide-spectrum mat...
ORGANISM(S): Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2020-05-07 | PXD018176 | Pride
Lc-msms E. coli Ovotransferrin Fusion lumos Dual ms2 Uvpd 213 nm Hsa Myoglobin Creatine kinase 
ProteomicsReconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1
Protein-protein interaction within the MGM holocomplex has been investigated by chemical cross-linking mass spectrometry using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) cross-linker. Although this analysis was not exhaustive, we observed crosslinks between Alp4 and Alp6 along the length of...
ORGANISM(S): Schizosaccharomyces pombe 
2019-05-27 | PXD012624 | Pride
“mgm” (mto/gamma/mozart) holocomplex Cross-linking 
ProteomicsMolecular Basis for CPC-Sgo1 Interaction: Implications for Centromere Localisation and Function of the CPCMolecular Basis for CPC-Sgo1 Interaction: Implications for Centromere Localisation and Function of the CPC
Sgo1 makes multipartite interactions with CPC subunits Previous studies have suggested that the Sgo1-CPC (Chromosomal Passenger Complex) interaction is mediated via the N-terminal coiled-coil of Sgo1 and Borealin. However, our structural data revealed that the very N-terminus of Sgo1 can interact wi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-29 | PXD028433 | Pride
Crosslinking mass spectrometry Cpc-sgo1 interaction 
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