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ProteomicsProteomic analysis of leucine effect on OMM protein turnover in HEK293 cells
This project is to investigate whether and how leucine affects endogenous OMM protein stability in HEK293 cells. Whole cell and crude mitochondria samples (with/without leucine and/or CHX treatment) were measured in this project.
ORGANISM(S): Homo sapiens (Human) 
2025-11-05 | PXD051398 | Pride
Protein turnover Outer mitochondrial membrane Leucine Mitochondrial enrichment 
ProteomicsInteractome of NDUFAF5-HA overexpressing cell line compared to mock
Proteomic analysis to assess the interactome of NDUFAF5-HA.
ORGANISM(S): Homo sapiens (Human) 
2023-05-08 | PXD040453 | Pride
Ndufaf5 
ProteomicsInteractome of NDUFAF8-HA overexpressing cell line compared to mock
Proteomic analysis to assess the interactome of NDUFAF8-HA.
ORGANISM(S): Homo sapiens (Human) 
2023-05-08 | PXD040495 | Pride
Ndufaf8 
ProteomicsWhole cell proteomics of NDUFAF8 knock-out cell line and NDUFAF5 knock-out cell line
Comparison of whole cell proteome of NDUFAF8 KO and NDUFAF5 KO to WT cell line
ORGANISM(S): Homo sapiens (Human) 
2023-05-08 | PXD040441 | Pride
Ndufaf5 Ndufaf8 
ProteomicsALR Couples IMS Redox and Heme Biosynthesis Beyond the Disulfide Relay
Denaturing pulldown of ALR-HA from HEK293 cells to identify the covalent interaction partners of ALR. One half of the samples were treated with DTT during the Co-IP protocol to verify that the interactions are disulfide related. Accordingly, no Mock-cell lines was used. One half of the samples from ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-16 | PXD075266 | Pride
Heme biosynthesis Oxidative protein folding Mitochondria 
ProteomicsDefinition of the ALR interactome by IP and AP under different lysis conditions
Interactome of overexpressed HA-tagged (2771, 2918) or Strep-tagged (3555, 3592) ALR in HEK293 FLP/IN/T-Rex cells vs control Mock under native (2771, 3592) or denaturing (2883, 3555) lysis conditions. For project 2771, an interactome of HA-tagged SOD2 was generated as additional negative control.
ORGANISM(S): Homo sapiens (Human) 
2026-04-16 | PXD064631 | Pride
Ims Mitochondrial disulphide relay Mitochondria  alr  cpox 
ProteomicsWhole cell proteome of cells lacking heme biosynthesis enzyme CPOX
Whole cell proteomics of a CRISPR/Cas9-generated HEK293 FLP/IN/T-Rex CPOX KO cell line in comparison to HEK293 FLP/IN/T-Rex WT cells and HEK293 FLP/IN/T-Rex CPOX KO cells complemented with CPOX-HA WT.
ORGANISM(S): Homo sapiens (Human) 
2026-04-16 | PXD064668 | Pride
Mitochondria  cpox  ims  heme biosynthesis 
ProteomicsInteraction with AK2 establishes AIFM1 as organizing platform in mitochondria
Interactome of overexpressed, HA-tagged AIFM1 in HEK293T AIFM1 KO background vs control (3797, 3593), Interactome of overexpressed, HA-tagged AIFM1 variants in HEK293T AIFM1 KO background vs control (3859), Comparison of whole cell extract after gelfiltration of HEK293T AIFM1 KO cell line vs WT
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD055617 | Pride
Aifm1 Ak2 Mitochondria 
ProteomicsMitochondrial calcium uniporter complex controls T‑cell‑mediated immune responses
Sustained and balanced calcium (Ca2+) increase upon T-cell receptor activation is a fundamental process that regulates essential T-cell functions including proliferation, clonal expansion and cytokine secretion. In this context, mitochondria play an important role and take up Ca2+ to support the ele...
ORGANISM(S): Homo sapiens (Human) 
2024-10-24 | PXD048682 | Pride
Mcu/autoimmunity/calcium/eae/metabolism/mitochondria/t-cell 
UnknownKinetic control by limiting glutaredoxin amounts enables thiol oxidation in the reducing mitochondrial intermembrane space.
Not available
S-EPMC4294668 | biostudies-literature
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