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ProteomicsMass spectrometric and bio-computational analysis of multiply charged RNase S gas phase complexes obtained by electrospray ionization from varying in-solution equilibrium conditions
In this project we investigate the influence of a solvent's composition on the stability of desorbed and multiply charged RNase S ions by analyzing gas phase dissociation reactions triggered by collision induced dissociation (CID) of multiply charged complex ions. RNase S was dissolved in ESI-comp...
ORGANISM(S): Bos taurus (Bovine) 
2021-11-23 | PXD027723 | Pride
Rnase s Item-two Off-line esi-ms Varying content of meoh co-solvent Different ph Complex stability 
GenomicsGenome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria
During transcription the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout budding yeast genome. In wild-type strains, R-loops were readily detected over...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-03 | E-GEOD-53420 | biostudies-arrayexpress
TranscriptomicsIdentification of RNAs that are physically and functionally associated with RNase P
Numerous RNAs copurify with RNase P and are affected by temperture sensitive mutations in conserved residues with the essential RNA and protein subunits. Specifically, RNase P physically interacts with ribosomal protein mRNAS and the intron-encoded box C/D snoRNAs. Keywords: RNA copurification, tem...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-GEOD-10514 | biostudies-arrayexpress
TranscriptomicsTranscriptome of Sinorhizobium meliloti RNase mutants
The ribonucleases (RNases) E and J are essential in Escherichia coli and Bacillus subtilis, respectively. Sinorhizobium meliloti contains both, the rne gene encoding RNase E and the rnj gene encoding RNase J. The transcriptomes of the S. meliloti Rm2011 wild type, and rne and rnj mutants were compar...
ORGANISM(S): Sinorhizobium meliloti 
2017-01-21 | E-MTAB-5435 | biostudies-arrayexpress
TranscriptomicsAccumulation of noncoding RNA due to an RNase P defect in Saccharomyces cerevisiae
To investigate the possible range of additional RNase P substrates in vivo a strand-specific, high-density microarray was used to analyze what RNA accumulates with a mutation in the catalytic RNA subunit of nuclear RNase P in Saccharomyces cerevisiae. A wide variety of noncoding RNAs were shown to a...
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-29 | E-MEXP-3140 | biostudies-arrayexpress
ProteomicsiTRAQ-based proteomic analysis of style proteins and S-RNase-related proteins in Huanghua and its homozygous S-genotype offspring
We used iTRAQ technology to investigate the protein expression characteristics of S-RNases in S-gene homozygous germplasm. By using MaxQuant to search the raw mass spectrometry data files against the protein database, a total of 30,503 identified spectra, 13,568 peptides, and 4,472 proteins were ide...
ORGANISM(S): Pyrus pyrifolia 
2024-01-26 | PXD043543 | Pride
S-rnase Style Itraq Pyrus Homozygous 
TranscriptomicsGene expression profiling of constitutively competent comR and proQ mutants and RNase R (rnr) mutant of L. pneumophila
Bacterial competence is a genetically programmed process that is triggered as a response to environmental stimuli. DNA uptake from the extracellular milieu by competence requires the expression of a complex machinery composed of a competence pseudopilus and a DNA translocase. In L. pneumophila, it ...
ORGANISM(S): Legionella pneumophila 
2010-05-20 | E-GEOD-12796 | biostudies-arrayexpress
TranscriptomicsIntroducing synthetic thermostable RNase inhibitors in single-cell RNA-seq
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, there has b...
ORGANISM(S): Mus musculus 
2024-07-29 | E-MTAB-13873 | biostudies-arrayexpress
ProteomicsThe proteome reveals serine/threonine kinase in pollen tube regulates S-RNase enzyme protein degradation in almond
Gametophytic self-incompatibility is the primary cause of low fruit set in almond. The mechanism of recognition that determines whether the gametophyte is successfully fertilized between pollen tube SCF (F-box-SSK1-Cul1-Rbx1) protein and pistil S-RNase protein during fertilization is unclear. In thi...
ORGANISM(S): Prunus dulcis 
2022-02-10 | PXD029128 | Pride
S-rnase Almond Mark2 Scf Gametophytic self-incompatibility 
ProteomicsComposition and RNA binding specificity of metazoan RNase MRP
Mechanistic studies have revealed ribonuclease (RNase) MRP, a conserved RNA-based enzyme, is crucial for the maturation of ribosomal RNA in eukaryotes. However, the composition and RNA substrate specificity of this multisubunit ribonucleoprotein complex in higher eukaryotes remain unknown. Here, we ...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-15 | PXD061658 | Pride
Nepro Ribonuclease (rnase) mrp C18orf21 Ip-ms 
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