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Human HeLa cells were treated with various functional siRNAs of different sequence targeting several genes (100 nM or 50 nM for 24 hours). There are two transfection groups - in the first, containing biological duplicates (A and B), the mock-transfected control samples are pooled as a Cy3 reference...
ORGANISM(S): Homo sapiens 
Anthocyanins are flavonoid compounds responsible for red/purple colours in the leaves, fruit and flowers of many plant species. They are produced through a multistep pathway which is controlled by MYB transcription factors. VvMYBA1 and VvMYBA2 activate anthocyanin biosynthesis in grapevine (Vitis vi...
ORGANISM(S): Vitis vinifera 
To identify the co-immunoprecipitating proteins of Rab27a following Rab27a pull down of B16-F1 mouse melanoma cells infected with adenovirus expressing GFP-hsSPIRE1.
ORGANISM(S): Mus musculus (Mouse) 
2020-05-15 | PXD018083 | Pride
Trimethylation of histone H3 lysine 27 (H3K27me3) plays a critical role in regulating gene expression during plant and animal development. We characterized the genome-wide distribution of H3K27me3 in five developmentally distinct tissues in maize plants of two genetic backgrounds, B73 and Mo17, rep...
ORGANISM(S): Zea mays 
Lipids comprise 70% of the myelin sheath, and autoantibodies against lipids may contribute to the demyelination that characterizes multiple sclerosis (MS). We used lipid antigen microarrays and lipid mass spectrometry to identify bona fide lipid targets of the autoimmune response in MS brain and an ...
ORGANISM(S): Homo sapiens 
Lipids comprise 70% of the myelin sheath, and autoantibodies against lipids may contribute to the demyelination that characterizes multiple sclerosis (MS). We used lipid antigen microarrays and lipid mass spectrometry to identify bona fide lipid targets of the autoimmune response in MS brain and an ...
ORGANISM(S): Homo sapiens 
Lipids comprise 70% of the myelin sheath, and autoantibodies against lipids may contribute to the demyelination that characterizes multiple sclerosis (MS). We used lipid antigen microarrays and lipid mass spectrometry to identify bona fide lipid targets of the autoimmune response in MS brain and an ...
ORGANISM(S): Homo sapiens 
The aim of the project was to identify proteins of the basal complex of Toxoplasma gondii using MyoJ as bait
ORGANISM(S): Toxoplasma gondii RH 
2022-11-01 | PXD035106 | Pride
Study of Trypanosoma ZMYND12’s ortholog (TbTAX-1) confirmed an axonemal localization of the protein and knockdown of TbTAX-1 using RNAi induced dramatic flagellar motility defects as observed in human. Co-immunoprecipitation assays and ultra-structure expansion microscopy in Trypanosoma brucei then ...
ORGANISM(S): Trypanosoma brucei 
2023-11-01 | PXD039470 | Pride
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