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ProteomicsAcetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids.
In this project we have studied human hepatoma cell line grown in 3D spheroids subjected to APAP (acetaminophen) treatment. Global protein expression analysis (label free) and analysis of cysteine oxidations (SNO/SOH TMT proteomics) have been combined to get insights in to the molecular response tri...
ORGANISM(S): Homo sapiens (Human) 
2018-10-18 | PXD003587 | Pride
Apap  3d cell culture  s-nitrosylation  s-sulfenylation  quantitative redox proteomics  toxicology  hepg2/c3a 
ProteomicsFlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS
High-resolution capillary chromatography is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We report a simple and low-cost FlashPack protocol for preparation of ultra-high performance capillary columns of > 50 cm length in less than 1 h using low-pressure equipment...
ORGANISM(S): Homo sapiens (Human) 
2019-01-21 | PXD009519 | Pride
Chromatography Capillary collumn packing Lc-msms 
ProteomicsProteomic analysis of phosphorylation, lysine acetylation and total protein changes across differentiating human muscle cells (TMT10plex).
We have carried out proteomic analysis of phosphorylation, lysine acetylation and protein abundance changes across a 6 day time course of human myoblasts differentiating into myotubes in cell culture.
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD032760 | Pride
Lc-ms/ms Muscle cell Acetylation Tmt10plex Stem cells Phosphorylation Muscle 
ProteomicsLabel free proteomics analysis of differentiating human muscle cells.
We have carried out systematic analysis of protein expression changes during differentiation of human myoblasts into myotubes over 6 days period in cell culture.
ORGANISM(S): Homo sapiens (Human) 
2020-05-20 | PXD018588 | Pride
Lc-ms/ms Stem cells Label free Muscle 
ProteomicsHypoxia response in barley - proteomics analysis
Hypoxia stress (<1% oxygen) followed by reoxygenation, is accompanied by formation of reactive oxygen species (ROS) and nitric oxide (NO), which act as signaling molecules, but also cause nitro-oxidative stress. The NO concentration is regulated by phytoglobins (formerly hemoglobins) in the NO cycle...
ORGANISM(S): Hordeum vulgare (Barley) 
2020-03-02 | PXD017103 | Pride
Barley Hypoxia Lc-ms/ms 
ProteomicsLabel-free mass spectrometry analysis of laminin with induced oxidations and chlorination’s facilitated with hypochlorous acid and myeloperoxidase treatments.
Basement membranes are specialized extracellular matrices that underlie arterial wall endothelial cells, with laminin being a key structural and biologically-active component. Hypochlorous acid (HOCl), a potent oxidizing and chlorinating agent, is formed in vivo at sites of inflammation, via the enz...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-05 | PXD022056 | Pride
Ecm Lc-ms/ms Oxidation Extracellular matrix proteins Inflammation. Chlorination Laminin 
ProteomicsLabel-free proteomic analysis of hepatocyte spheroids fed physiologically relevant levels of palmitic acid and oleic acid to mimic non-alcoholic fatty liver disease.
We have grown hepatocyte spheroids for 21 days following an established protocol. We have carried out proteomic analysis of the difference between control and treated (Fatty acids) spheroids during seven days (5 timepoints at day 0, 1, 2, 4 and 7). Samples were digesting using filter aided sample pr...
ORGANISM(S): Homo sapiens (Human) 
2021-11-24 | PXD029200 | Pride
Lc-ms/ms Liver 3-dimensional cell culture Hepatocyte Spheroids Rotary bioreactor Label-free Non-alcoholic fatty liver disease Hepg2/c3a Nafld 
ProteomicsProteomics of non-host response in barley
Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley (Hordeum vulgare L.) plants to infection by powdery mildew, Blumeria graminis f. sp. Triti...
ORGANISM(S): Hordeum vulgare (Barley) Blumeria graminis f. sp. tritici 
2020-08-18 | PXD015089 | Pride
Phytoglobin/hemoglobin overexpression Proteomics Mildew Barley 
TranscriptomicsTranscription factor cooperativity in early adipogenic hotspots and super-enhancers
Here we have used a combination of advanced proteomics and genomics approaches to investigate the extent and mechanisms of transcription factor cross-talk at genomic hotspots. We identify ~12,000 transcription factor hotspots in the early phase of adipogenesis, and we find evidence of both simultane...
ORGANISM(S): Mus musculus 
2014-04-17 | E-GEOD-56872 | biostudies-arrayexpress
ProteomicsGeneration of aggregates of alpha-lactalbumin by UV-B light exposure
Whey proteins are widely used as ingredients in the form of aggregates to obtain certain functionalities in food applications. The aim of this study was to understand how UV illumination generates aggregates of alpha-lactalbumin (a-LA) as an alternative to heat treatments traditionally used for indu...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) 
2019-12-16 | MSV000084695 | MassIVE
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