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ProteomicsIdentification of proteins interacting with translating RPAC reporter mRNAs (FGH17)
To discover new regulators of Regulatory Particle Assembly Chaperone (RPAC) translation, we identified RNA-binding proteins (RBPs) with increased recruitment to translating WT FGH17 mRNAs compared to non-translatable FGH17-40ntΔ mRNAs in vivo. To this end, we first treated yeast cells with rapamycin...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-05-04 | PXD027655 | Pride
Proteasome assembly chaperone translation 
ProteomicsPhospho-regulation of Cdc48/p97 cofactor protein Shp1/p47 in budding yeast
Shp1/p47 is a cofactor of the Cdc48/p97 complex involved in the energy-dependent segregation of intracellular aggregates and multiprotein complexes. We identified two serine residues (S108 and S315) in the S. Cerevisiae protein Shp1 which increase in phosphorylation upon various cell stresses such a...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-30 | PXD035819 | Pride
Ubx domain Stress Phosphorylation Torc1 Protein degradation Ubx1 Proteostasis 
ProteomicsIdentification of Zuo1 interactors upon rapamycin stress
Proteostasis is tightly regulated by TORC1 to meet cellular requirements. Upon TORC1 inhibition, degradative activity is increased, and protein synthesis is reduced through inhibition of translation initiation, to maintain cell viability. Here, we show that the ribosome-associated complex (RAC)/Ssb ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-09-11 | PXD039550 | Pride
Lc-msms Yeast Tmt-labelling Immunoprecipitation Co-chaperones 
UnknownThe ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition
Not available
S-SCDT-10_15252-EMBJ_2022113240 | biostudies-other
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