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ProteomicsQuantitative Proteomics Analysis of Rat Brown Adipose Tissue Based on TMT Labelling
This study employed mouse brown adipose tissue as the sample, utilising TMT labelling combined with LC-MS/MS technology to conduct qualitative identification and relative quantitative analysis of its proteome. By comparing protein expression profiles between different treatment groups (control vs. c...
ORGANISM(S): Rattus norvegicus (Rat) 
2026-08-27 | PXD071045 | Pride
Lc-ms/ms Rat Brown adipose tissue 
ProteomicsQuantitative N-terminome of N-end rule mutant in Arabidopsis
According to the /N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Here, we undertook a quantitative proteomics study of N-end rule mutant prt6, to investigate the impact of this pathway on the etiolated seedli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-10-27 | PXD006450 | Pride
N-terminome Arabidopsis N-end rule mutant etiolated seedlings tmt- labelling tails 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsMass spectrometry analysis of estrogen receptor α -interacting proteins.
Quantitative mass spectrometry analysis using tandem mass tags (TMT) labelling of estrogen receptor α (ERα) pull downs. Co-immunoprecipitated proteins using a anti Erα antibody were compared quantitatively with material recovered in a mock to identify ERα-interacting proteins.
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD025018 | Pride
Estrogen receptor Mrna binding Tmt Immunoprecipitation 
ProteomicsGlobal phosphoproteome of Leishmania mexicana developmental stages
Using a combination of phosphoproteome enrichment and tandem mass tag (TMT) labelling-based quantitative proteomic mass spectrometry (MS), we robustly identified and quantified 1,833 phosphorylated proteins across three life cycle stages of Leishmania mexicana (L. mexicana) parasite. Protein kinase ...
ORGANISM(S): Leishmania mexicana MHOM/GT/2001/U1103 
2023-10-04 | PXD043002 | Pride
Phosphoproteome Tmt labelling Leishmania 
ProteomicsProteome of the mantle tissue of the Yesso scallop
Protiome of the mantle tissue of the Yesso scallop (Patinopecten yessoensis) is analysed based on the TMT labelling technology
ORGANISM(S): Mizuhopecten Yessoensis 
PXD033456 |
ProteomicsTranscriptome-wide identification of coding and noncoding RNA-binding proteins defines the extended RNA interactome of Leishmania mexicana
Non-poly(A) RNA molecules including noncoding RNAs (ncRNAs) comprise the major portion of the total transcribed molecules in the cell. In addition to the mRNAs the ncRNAs also function as ribonucleoprotein particles (RNPs) and carry out biological functions including synthesis of new proteins, RNA p...
ORGANISM(S): Leishmania mexicana MHOM/GT/2001/U1103 
2022-01-14 | PXD028092 | Pride
Leishmania Rna-binding proteins 
ProteomicsProteomics of Metronidazole Resistance in three seminal lines of Giardia duodenalis
Metronidazole (Mtz) is the frontline chemotherapeutic for a variety of anaerobic pathogens, including the gastroenterinal parasitic protist, Giardia duodenalis. However, treatment failure and clinical resistance is common and increasing. In Giardia, in vitro drug-resistant lines allow controlled exp...
ORGANISM(S): Giardia lamblia ATCC 50803 
2018-02-21 | PXD007183 | Pride
Proteomics Tmt labelling Giardia 
ProteomicsEfficient removal of TMT-labelling by-products in proteomic samples by methylamine  
Comparing the performance of methylamine and hydroxylamine on phospho-peptide analysis, particularly in relation to the identification of more complex dose-dependent patterns.
ORGANISM(S): Homo sapiens (Human) 
2025-03-17 | PXD056908 | Pride
Proteomics Over-labelling Tmt-labeling Phosphoproteomics 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
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