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ProteomicsRPA promotes processive ssDNA digestion by Dna2 via their bimodal interaction in Saccharomyces cerevisiae
Single-stranded DNA (ssDNA) widely exists as intermediates in DNA metabolic pathways. The ssDNA binding protein, RPA, not only protects the integrity of ssDNA, but also directs the downstream factor that signals or repairs the ssDNA intermediate. However, it remains unclear how these enzymes/factors...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-22 | PXD028637 | Pride
Ssdna Saccharomyces cerevisiae Dna2 Rpa 
ProteomicsMechanism of RPA phosphocode priming and tuning by Cdk1/Wee1 signaling circuit
Replication protein A (RPA) is a heterotrimeric single-strand DNA binding protein that is integral to DNA metabolism. Segregation of RPA functions in response to DNA damage is fine-tuned by hyperphosphorylation of the RPA32 subunit that is dependent on Cyclin-dependent kinase (Cdk)-mediated priming ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD068080 | Pride
Rpa domain configurations Rpa phosphorylation Cdk1 Wee1 Cell cycle 
ProteomicsAntagonistic roles of canonical and alternative-RPA in disease-associated tandem CAG repeat instability
Ongoing CAG expansions in Spinocerebellar ataxia type 1 (SCA1) and Huntington disease (HD) brains exacerbate disease.  Expansions involve aberrant repair of mutagenic slipped-DNAs, formed from single-stranded DNAs.  Whether singlestranded DNA-binding proteins prevent or facilitate expansions is unkn...
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD044158 | Pride
Cag repeat expansion diseases  spinocerebellar ataxia type 1 (sca1)  huntington's disease (hd)  somatic cag repeat expansions  rpa (single-strand dna binding protein  alternative-rpa (alt-rpa)  dna repair Slipped-dnas  post-mortem brains Sca1 mice 
GenomicsRPA accumulation during class switch recombination represents 5'-3' DNA end resection during the S-G2/M phase of the cell cycle
AID promotes chromosomal translocations by inducing DNA double-strand breaks (DSBs) at immunoglobulin (Ig) genes and oncogenes in G1. RPA is a ssDNA-binding protein that associates with resected DSBs in the S phase and facilitates the assembly of factors involved in homologous repair (HR) such as R...
ORGANISM(S): Mus musculus 
2013-01-02 | E-GEOD-42451 | biostudies-arrayexpress
TranscriptomicsSub1 and RPA associate with RNA Polymerase II at different stages of transcription
Single stranded DNA binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. Quantitative proteomic analysis of RNA polymerase II (RNApII) pre-initiation complexes (PICs) identified Sub1 and the Replication Protein A complex (RPA), both ...
ORGANISM(S): Saccharomyces cerevisiae 
2011-09-26 | E-GEOD-32416 | biostudies-arrayexpress
TranscriptomicsRNA processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA.
Eukaryotic cells respond to DNA double-strand breaks (DSBs) by activating a checkpoint that depends on the protein kinases Tel1/ATM and Mec1/ATR. Mec1/ATR is activated by RPA-coated single-stranded DNA (ssDNA), which arises upon nucleolytic degradation (resection) of the DSB. Emerging evidences indi...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-20 | E-GEOD-63444 | biostudies-arrayexpress
ProteomicsPhase separation properties of RPA combine high-affinity ssDNA binding with dynamic condensate functions at telomeres
RPA has been shown to protect single-stranded DNA (ssDNA) intermediates from instability and breakage. RPA binds ssDNA with sub-nanomolar affinity, yet dynamic turnover is required for downstream ssDNA transactions. How ultrahigh-affinity binding and dynamic turnover are achieved simultaneously is n...
ORGANISM(S): Homo sapiens (Human) 
2023-03-15 | PXD036935 | Pride
Alternative lengthening of telomeres (alt) Replication protein a (rpa) Nuclear organization Biomolecular condensates Genome stability Single-stranded dna (ssdna) 
GenomicsGelatoporia subvermispora strain:RPA
Ceriporiopsis subvermispora RPA Gene Expression Profiling - GluRPA1 transcriptome
PRJNA1389414 | ENA
GenomicsGelatoporia subvermispora strain:RPA
Ceriporiopsis subvermispora RPA Gene Expression Profiling - GluRPA3 transcriptome
PRJNA1389183 | ENA
GenomicsGelatoporia subvermispora strain:RPA
Ceriporiopsis subvermispora RPA Gene Expression Profiling - AviRPA1 transcriptome
PRJNA1389585 | ENA
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