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TranscriptomicsArabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability
RNA sequencing performed on the root tips of wee1-1 and wee1-1/rnh2a mutants, treated for 24h with or without 2mM hydroxyurea.
ORGANISM(S): Arabidopsis thaliana 
2016-05-20 | E-MTAB-2590 | biostudies-arrayexpress
TranscriptomicsTransfer of 5d old Col-0 and WEE1 KO seedlings to control medium for 0h-5h-24h
Col-0 and WEE1KO (wee1-1 and wee1-2) were germinated on control medium on a nylon mesh and transferred 5 days after germination to control medium. Samples were harvested at three different time points after transfer: 0 h, 5 h, and 24 h. All sampling points were performed in four independent experime...
ORGANISM(S): Arabidopsis thaliana 
2011-07-24 | E-MEXP-3048 | biostudies-arrayexpress
TranscriptomicsTRRAP-mediated regulation of Wee1
The G2 DNA damage checkpoint inhibits Cdc2 and mitotic entry through the dual regulation of Wee1 and Cdc25 by the Chk1 effector kinase. Up-regulation of Chk1 by mutation or overexpression bypasses the requirement for up-stream regulators or DNA damage to promote a G2 cell cycle arrest. We screened i...
ORGANISM(S): Schizosaccharomyces pombe 
2010-04-11 | E-GEOD-12674 | biostudies-arrayexpress
TranscriptomicsTransfer of 5d old Col-0 and WEE1 KO seedlings to medium supplemented with 2mM HU for 0h-5h-24h
Col-0 and WEE1KO (wee1-1 and wee1-2) were germinated on control medium on a nylon mesh and transferred 5 days after germination to medium supplemented with 2 mM HU. Samples were harvested at three different time points after transfer: 0 h, 5 h, and 24 h. All sampling points were performed in four in...
ORGANISM(S): Arabidopsis thaliana 
2011-07-24 | E-MEXP-3053 | biostudies-arrayexpress
TranscriptomicsGenome-wide CRISPR-Cas9 screens reveal loss of redundancy between PKMYT1 and WEE1 in Glioblastoma stem-like cells
To identify new therapeutic targets for Glioblastoma (GBM), we performed genome-wide CRISPR-Cas9 "knockout" (KO) screens in patient-derived GBM stem-like cells (GSCs) and human neural stem/progenitors (NSCs), non-neoplastic stem cell controls, for genes required for their in vitro growth. ...
ORGANISM(S): Homo sapiens 
2016-02-15 | E-GEOD-70038 | biostudies-arrayexpress
ProteomicsMacrophage WEE1 promotes atherosclerosis via directly phosphorylating NF-κB p65 subunit and inducing inflammatory response
Background: Atherosclerosis leads high mortality, highlighting an urgent need for new therapeutic strategies. Protein kinases orchestrate multiple cellular events in atherosclerosis and may provide new therapeutic targets for atherosclerosis. Here, we identified a protein kinase, WEE1 G2 checkpoint ...
ORGANISM(S): Homo sapiens (Human) 
2024-03-25 | PXD050932 | Pride
Human 
TranscriptomicsWee1 Inhibition sensitizes prostate cancer cells to Hsp90 inhibitor to activate the intrinsic apoptotic pathway
Heat shock protein 90 (Hsp90) is an essential evolutionarily conserved molecular chaperone in eukaryotes. Cancer cells rely on Hsp90 to chaperone activated oncoproteins, and its involvement in numerous signaling pathways makes it an attractive target for drug development. Surprisingly, however, the ...
ORGANISM(S): Homo sapiens 
2013-01-10 | E-GEOD-38972 | biostudies-arrayexpress
ProteomicsDevelopment and Characterization of a Wee1 Kinase Degrader
The G1/S cell-cycle checkpoint is frequently dysregulated in cancer, leaving cancer cells particularly reliant on a functional G2/M checkpoint to prevent excessive DNA damage. Inhibiting regulators of the G2/M checkpoint can therefore drive cancer cells into premature mitosis, ultimately causing cel...
ORGANISM(S): Homo sapiens (Human) 
2019-11-18 | PXD015513 | Pride
Cancer Wee1 Crl4 Crbn Protac Ubiquitin 
GenomicsChIP-seq analysis of RNA polymerase II occupancy in wt, wee1-50 and cdc25-22 fission yeast cells
This experiment aimed at understanding the correlation between RNA polymerase II occupancy and cell size. For this, we used three strains of increasing average size (wee1-50 < wt < cdc25-22) and performed ChIP-seq experiments using anti-CTD antibodies.
ORGANISM(S): Schizosaccharomyces pombe 
2020-01-01 | E-MTAB-8522 | biostudies-arrayexpress
TranscriptomicsFS001: Aberrant gene expression in dogs with portosystemic shunts
Background: Congenital portosystemic shunts are developmental anomalies of the vascular system that cause portal blood to bypass the liver. Large-breed dogs are reported to have a predisposition for intrahepatic portosystemic shunts (IHPSS) and small-breed dogs a predisposition for extrahepatic port...
ORGANISM(S): Canis lupus familiaris 
2013-03-01 | E-GEOD-39005 | biostudies-arrayexpress
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