Sort   by:  
 Page size 
ProteomicsMechanism of RPA phosphocode priming and tuning by Cdk1/Wee1 signaling circuit
Replication protein A (RPA) is a heterotrimeric single-strand DNA binding protein that is integral to DNA metabolism. Segregation of RPA functions in response to DNA damage is fine-tuned by hyperphosphorylation of the RPA32 subunit that is dependent on Cyclin-dependent kinase (Cdk)-mediated priming ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD068080 | Pride
Rpa domain configurations Rpa phosphorylation Cdk1 Wee1 Cell cycle 
ProteomicsPHOSPHORYLATION-DEPENDENT ASSOCIATION OF WRN WITH RPA IS REQUIRED FOR RECOVERY OF REPLICATION FORKS STALLED AT SECONDARY DNA STRUCTURES
The WRN protein mutated in the premature aging disorder Werner syndrome is vital for metabolism of perturbed replication forks. Replication Protein A (RPA) robustly enhances WRN helicase activity in specific cases when tested in vitro. However, the significance of RPA-binding to WRN in vivo has rema...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095749 | MassIVE
ProteomicsBreak-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32
Break-induced replication (BIR) mediates single-ended DNA double-strand break (DSB) repair. Upon strand invasion into a homologous repair template, BIR is catalysed by a minimal replisome comprising PCNA, DNA polymerase delta, and the Pif1 helicase. Here, we identify an interaction between Polymeras...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-06-15 | PXD074712 | Pride
Cdc5 Break-induced replication Vanquish neo uhplc Ddk Orbitrap exploris 480 Pol32 Cka2 Saccharomyces cerevisiae 
ProteomicsO-GlcNAcylation of RPA2 at S4/S8 antagonizes phosphorylation and regulates checkpoint activation during replication stress
O-linked N-acetylglucosamine (O-GlcNAc) is the only mono-saccharide modification occurring in the cytoplasm, nucleus and mitochondria. Recent advent of the mass spectrometry technology has enabled identification of abundant O-GlcNAc transferase (OGT) substrates in diverse biological processes, such ...
ORGANISM(S): Homo Sapiens 
PXD053321 |
ProteomicsPhase separation properties of RPA combine high-affinity ssDNA binding with dynamic condensate functions at telomeres
RPA has been shown to protect single-stranded DNA (ssDNA) intermediates from instability and breakage. RPA binds ssDNA with sub-nanomolar affinity, yet dynamic turnover is required for downstream ssDNA transactions. How ultrahigh-affinity binding and dynamic turnover are achieved simultaneously is n...
ORGANISM(S): Homo sapiens (Human) 
2023-03-15 | PXD036935 | Pride
Alternative lengthening of telomeres (alt) Replication protein a (rpa) Nuclear organization Biomolecular condensates Genome stability Single-stranded dna (ssdna) 
UnknownRole of RPA Phosphorylation in the ATR-Dependent G2 Cell Cycle Checkpoint.
Not available
S-EPMC10742774 | biostudies-literature
UnknownRPA Phosphorylation Inhibits DNA Resection.
Not available
S-EPMC6625828 | biostudies-literature
UnknownPhosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure.
Not available
S-EPMC11772831 | biostudies-literature
GenomicsGenome wide localization of CTCF in activated mouse B cells
Identify CTCF binding sites in activated mouse B cells. Single ChIP experiment
ORGANISM(S): Mus musculus 
2011-11-19 | E-GEOD-33819 | biostudies-arrayexpress
UnknownAlteration of replication protein A binding mode on single-stranded DNA by NSMF potentiates RPA phosphorylation by ATR kinase.
Not available
S-EPMC10450186 | biostudies-literature
Sort   by:  
 Page size