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ProteomicsTRX1-enhanced CAR T cells retain their antitumor functions under pro-oxidative conditions
Immunotherapies with CAR-T cells achieve remarkable success, especially against B-cell malignancies. However, their activity against non-hematopoietic solid tumors is very limited. One central cause of this failure may be that a pro-oxidative tumor microenvironment can downmodulate the activation an...
ORGANISM(S): Homo sapiens (Human) 
2022-11-23 | PXD031073 | Pride
Car t cells Ros Immunosuppression Thioredoxin 1 Cancer immunotherapy Redox regulation 
ProteomicsIdentification of ELC interacting proteins in zebrafish heart
The essential myosin light chain (ELC) is part of the cardiac myosin motor regulating the contractility of the heart. Recently, we have demonstrated ELC phosphorylation to be responsible not only for basal maintenance of cardiac contractility, but also for the adaptation of sarcomere function to inc...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2022-07-24 | PXD016357 | Pride
Myosin light chain Elc Co-immunoprecipitation Phosphorylation 
ProteomicsAnalysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins
The myosin light chain protein family consists of two classes, the regulatory myosin light chains (RLC) and the essential myosin light chains (ELC). Functionally, the MLC proteins are directly involved in sarcomeric activity and force transmission contributing to cardiac contractility. Besides regul...
ORGANISM(S): Homo sapiens (Human) 
2022-07-24 | PXD033908 | Pride
Cardiomyocytes Essential myosin light chains (elc) Phosphorylation Regulatory myosin light chains (rlc) 
ProteomicsAnalysis of myosin light chain migration pattern in 2D gels of human left ventricular proteins - proximity labeling
NIMA-related kinase 9 (NEK9) was identified to interact with the essential myosin light chain (ELC). To validate these findings ascorbate peroxidase (APEX) catalyzed proximity labeling (Hung et al. Nat Protoc. 2016) was performed. To do so, human ELC fused to APEX was overexpressed in HEK293 cells. ...
ORGANISM(S): Homo sapiens (Human) 
2022-07-24 | PXD034115 | Pride
Bioid Nek9 Elc 
ProteomicsMass-spectrometry based quantification of HYPK, NAA10 and NAA15 protein steady-state levels in Arabidopsis thaliana mutants carrying a T-DNA insertion in the HYPK gene.
N-alpha terminal acetylation is one of the major protein modifications in eukaryotes carried out by distinct Nats (N-alpha acetyltransferases). The core NatA complex is composed of the catalytic NAA10 and the auxiliary NAA15 subunit and co-translationally acetylates majority of the cytosolic protein...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-12 | PXD023599 | Pride
Arabidopsis thaliana Hypk Nata complex N-alpha terminal acetylation 
ProteomicsCellular responses in yeast proteostasis mutants affected in diverse branches of the protein quality control system
Protein quality controls (PQC) systems rely on three main activities to prevent the accumulation of misfolded proteins upon stress conditions and aging: refolding, degradation and sequestration. In Saccharomyces cerevisiae the Hsp70 chaperone system plays a central role in protein refolding, while d...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-10 | PXD037835 | Pride
Chaperone Sequestrases Hsp70 Proteasome Proteostasis 
Proteomics: Interacting partners of Trypanosoma brucei CPSF73 in the presence of mRNA processing inhibitor, AN7973
T. brucei CPSF73 endogenously TAP-tagged at the C-terminal was affinity purified after treating the cells for 15 min with AN7973 at 10x EC50. Three technical replicates were used. 3 technical replicates of untreated cells served as controls. Inducible GFP-TAP with and without treatment served as add...
ORGANISM(S): Trypanosoma brucei 
2022-10-19 | PXD033513 | Pride
An7973 Tap Cpsf73 
Proteomicsα-catenin interacts with YAP/FoxM1/TEAD-induced CEP55 and fosters liver cancer cell migration
Aberrant expression of adherens junction (AJ) proteins is frequently observed in human cancers. However, how these factors contribute to tumorigenesis is poorly understood and for some factors such as α‐catenin contradicting data has been described. Systematic analysis of TCGA expression data derive...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD039222 | Pride
Adherens junctions Forkhead box m1 Yes-associated protein Hippo pathway Hepatocellular carcinoma 
ProteomicsQuantification of the translatome in wild type Arabidopsis and amiNAA10 plants
Wild type Arabidopsis Col-0 plants and mutants depleted of the catalytic subunit of the ribosome-associated N-acetyltransferase A (NatA) complex (amiNAA10) were grown on soil for six weeks under short-day conditions (8h light, light intensity: 100 µE, 21 °C/18 °C temperature (day/night), 50 % humidi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD024328 | Pride
Arabidopsis thaliana Co-translational protein modification N-acetyltransferase a N-terminal protein acetylation Translation 
ProteomicsQuantification of poly-ubiquitinated proteins in wild type Arabidopsis and amiNAA10 plants
Wild type Arabidopsis Col-0 plants and mutants depleted of the catalytic subunit of the ribosome-associated N-acetyltransferase A (NatA) complex (amiNAA10) were grown on soil for six weeks under short-day conditions (8h light, light intensity: 100 µE, 21 °C/18 °C temperature (day/night), 50 % humidi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD024329 | Pride
Arabidopsis thaliana Co-translational protein modification Ubiquitin-proteasome system N-acetyltransferase a Post-translational protein modification N-terminal protein acetylation Protein degradation N-degron pathways Nonac-x²/n-degron 
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