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UnknownTandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors.
Not available
S-EPMC7854218 | biostudies-literature
ProteomicsYorkie and Bonus protein interactome-Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye
To identify novel regulators of the Hippo pathway, we performed affinity purification-mass spectrometry (AP-MS) using Drosophila embryos overexpressing Yki-EGFP with a ubiquitous driver da-GAL4, as well as cultured S2 cells expressing Yki-SBP. We identified the core Hippo pathway components, multipl...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-01-17 | PXD027934 | Pride
Embryo Drosophila Yki S2 cell Bon Lc-ms/ms 
UnknownEngineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.
Not available
S-EPMC4583386 | biostudies-literature
ProteomicsRPM-1 in vivo affinity-purification proteomics using C. elegans
To uncover putative RPM-1 protein-protein interactions, we performed a comprehensive series of in vivo affinity-purification proteomics experiments using C. elegans. We utilized integrated transgenes that express RPM-1 fused with a Protein G::Streptavidin binding peptide (GS) affinity tag expressed ...
ORGANISM(S): Caenorhabditis elegans 
2024-11-19 | PXD051783 | Pride
C. elegans Rpm-1 
ProteomicsAffinity Purification experiments for 24 human Bromodomain proteins involved in DNA repair and genome integrity (part 2 of 2)
Bromodomain proteins (BRD) are key chromatin regulators of genome function and stability, as well as therapeutic targets in cancer. In our associated publication (doi:10.1101/gad.331231.119) we systematically delineate the contribution of human BRD proteins for genome stability and DNA double-strand...
ORGANISM(S): Homo sapiens (Human) 
2019-12-12 | PXD016673 | Pride
R-loops Dna repair Human Bromodomain Chromatin Ap-ms Dna damage response Homologous recombination 
ProteomicsAffinity Purification experiments for 24 human Bromodomain proteins involved in DNA repair and genome integrity (part 1 of 2).
Bromodomain proteins (BRD) are key chromatin regulators of genome function and stability, as well as therapeutic targets in cancer. In our associated publication (doi:10.1101/gad.331231.119) we systematically delineate the contribution of human BRD proteins for genome stability and DNA double-strand...
ORGANISM(S): Homo sapiens (Human) 
2019-12-12 | PXD016700 | Pride
R-loops Dna repair Human Bromodomain Chromatin Ap-ms Dna damage response Homologous recombination 
UnknownProtocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells.
Not available
S-EPMC9304681 | biostudies-literature
ProteomicsNetwork of interactions between ZIKA virus non-structural proteins and human host proteins
The Zika virus (ZIKV) is a mosquito-borne Flavivirus and can be transmitted through an infected mosquito bite or through human-to-human interaction by sexual activity, blood transfusion, breastfeeding or perinatal exposure. After the 2015-2016 outbreak in Brazil, a strong link between ZIKV infection...
ORGANISM(S): Homo sapiens (Human) 
2020-06-18 | PXD014961 | Pride
Zikv Non-structural viral proteins Network Protein-protein interaction 
UnknownThe structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.
Not available
S-EPMC3640474 | biostudies-literature
ProteomicsMSec interactome - The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec
Tunneling NanoTubes (TNTs) are membrane conduits that mediate long distance intercellular crosstalk in several organisms and play vital roles during development, pathogenic transmission and cancer metastasis. However, the molecular mechanisms of TNT formation and function remain poorly understood. T...
ORGANISM(S): Homo sapiens (Human) 
2019-03-15 | PXD012441 | Pride
Human cellular interactome Esi-lc-ms Sbp affinity purification 
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