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This project used a custom-built capillary electrophoresis (CE) mass spectrometry (MS) platform to demonstrate the scalable analysis of proteins from single cells of varying sizes and protein content in different vertebrate biological models. Neural tissue fated giant cells were identified in cleava...
ORGANISM(S): Mus musculus (Mouse) Xenopus laevis (African clawed frog) 
2022-03-02 | PXD031955 | Pride
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luc...
ORGANISM(S): Mus musculus 
A Scalable Epitope Tagging Approach for High Throughput ChIP-seq Analysis ChIP-seq comparison between CRISPR editing cells using epitope antibody and non-editing cells using endogeneous TF antibody
ORGANISM(S): Homo sapiens 
Scalable Agricultural Microbiome Sampling: Operational Definitions, Pooling Strategies, and Preservation Methods
Advances in single-cell genomics enable commensurate improvements in methods for uncovering lineage relations among individual cells. Current sequencing based methods for cell lineage analysis depend on low resolution bulk analysis or rely on extensive single cell sequencing which is not scalable an...
ORGANISM(S): Homo sapiens 
In this study, we present a robust and scalable sample preparation protocol based on chemical lysis with trifluoroacetic acid (TFA), adapted from the SPEED method and optimized for human skin samples. The protocol simplifies tissue lysis while selectively leaving ECM scaffolds largely insoluble. As ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD066029 | Pride
Single-cell genomics is critical for understanding cellular heterogeneity in cancer, but existing library preparation methods are expensive, require sample preamplification and introduce coverage bias. Here we describe direct library preparation (DLP), a robust, scalable, and high-fidelity method th...
A generic, cost-effective and scalable cell lineage analysis platform
Scalable production of human cortical organoids using a biocompatible polymer
Stavrum2013 - Tryptophan Metabolism in Liver This model is described in the article: Model of tryptophan metabolism, readily scalable using tissue-specific gene expression data. Stavrum AK, Heiland I, Schuster S, Puntervoll P, Ziegler M. J. Biol. Chem. 2013 Nov; 288(48): ...
2024-09-02 | BIOMD0000000602 | BioModels
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