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miRNA profiling has been performed on normal mature B cells at different stages of differentiation (naive, germinal center and memory). The main goal is to identify miRNA signatures associated with differentiation stages of normal B cells. Keywords: miRNA, normal mature B cells Cells were isolated...
ORGANISM(S): Homo sapiens 
The project aims to evaluate the contribution of ADAR1 RNA editing to B cell lymphomagenesis, specifically in diffuse large B cell lymphoma (DLBCL). Within our DLBCL cohort, RNA editing targets transcripts within known lymphoma-driving pathways such as apoptosis, p53 and NF-kB signaling, as well as ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD041549 | Pride
Preparation of nuclear extracts

D. melanogaster Schneider cells were prepared as previously described by Andrews and Faller (1991). Briefly, in a typical preparation, 8x106 cells (grown in Schneider medium plus 10% fetal calf serum; GIBCO-BRL) were pelleted and resuspended in 1,5 mL cold PBS1...
ORGANISM(S): Drosophila melanogaster 
As part of the ICGC, the ICGC MMML-Seq project performed whole genome sequencing of a rare Burkitt lymphoma subtype with precursor B-cell immunophenotype. Analysis were performed in concordance with the guidelines of the ICGC.
Small-RNA profiles were generated for normal mature germinal center (GC) B cells and GC-derived lymphomas. Normal and tumor samples are derived from different donors.
ORGANISM(S): Homo sapiens 
Six different endogenous Drosophila proteins (all potential export factors) have been inactivated using RNAi and leptomycin B (LMB) treatments. Five different endogenous Drosophila proteins (all potential export factors) have been targeted by RNAi. The effect on mRNA export is analyzed by monitoring...
ORGANISM(S): Drosophila melanogaster 
The centromere-specific Histone H3-variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse-induction of CENH3 (Drosophila CID) in Schneider S2 cells incorporates into noncentromeric regions and generates CID islands ...
ORGANISM(S): Drosophila melanogaster 
Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed.

We performed a genome-wide mapping o...
ORGANISM(S): Drosophila melanogaster 
ChIP-chip analysis of GAGA factor and NELF in Drosophila S2 cells
ORGANISM(S): Drosophila melanogaster 
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