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ProteomicsIncreasing proteome depth while maintaining quantitative precision in short gradient data-independent acquisition proteomics
The combination of short liquid chromatography (LC) gradients and data independent acquisition (DIA) by mass spectrometry (MS) has proven its huge potential for high-throughput proteomics. However, the optimization of isolation window schemes resulting in a certain number of data points per peak (DP...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2023-05-10 | PXD036451 | Pride
Isolation windows Dia Multi-species Short-gradient 
ProteomicsPerchlorate-Specific Proteomic Stress Responses of Debaryomyces hansenii Could Enable Microbial Survival in Martian Brines
If life exists on Mars, it would face several challenges including the presence of perchlorates, which destabilize biomacromolecules by inducing chaotropic stress. However, little is known about perchlorate toxicity for microorganism on the cellular level. Here we present a proteomic investigation o...
ORGANISM(S): Debaryomyces hansenii 
2023-06-06 | PXD033237 | Pride
Debaryomyces hansenii Astrobiology Mars Martian brines Perchlorate Stress response 
ProteomicsSample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid Extraction
We report a universal detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Bacillus cereus ATCC 10987 Escherichia coli Staphylococcus aureus 
2019-11-25 | PXD011189 | Pride
Speed Sample preparation Quantitative proteomics 
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