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ProteomicsFP-biotin enrichment of serine hydrolases in Enterococcus faecium
In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of en...
ORGANISM(S): Enterococcus faecium Bacteria 
2024-06-22 | PXD048798 | Pride
Lc-msms Enterococcus faecium Activity-based protein profiling Fp-biotin Serine hydrolases 
ProteomicsActivity-Based Protein Profiling of Human and Plasmodium Serine Hydrolases and Interrogation of Potential Antimalarial Targets
Malaria remains a global health issue requiring the identification of novel therapeutic targets to combat drug resistance. Metabolic serine hydrolases are druggable enzymes playing essential roles in lipid metabolism. However, very few have been investigated in malaria-causing parasites. Here, we us...
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum (isolate 3D7) 
2022-10-14 | PXD035891 | Pride
Abp Abpp Plasmodium falciparum Serine hydrolase Malaria Chemical proteomics Fluorophosphonate 
ProteomicsMixed Alkyl/Aryl Phosphonates Identify Metabolic Serine Hydrolases as Antimalarial Targets
Malaria, caused by Plasmodium falciparum, remains a significant health burden. The barrier for developing anti-malarial drugs is the ability of the parasite to rapidly generate resistance. We previously demonstrated that Salinipostin A (SalA), a natural product, potently kills parasites by inhibitin...
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum (isolate 3D7) 
2024-07-15 | PXD051499 | Pride
Plasmodium Lipid metabolism Serine hydrolases Activity-based probes 
ProteomicsProtease activity profiling of ovarian clear cell carcinoma
Ovarian clear cell carcinoma (OCCC) is an understudied poor prognosis subtype of ovarian cancer lacking in effective molecularly targeted therapies; efforts to define drivers of OCCC malignancy may lead to new therapeutic targets and approaches. Among potential targets are secreted proteases, enzyme...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-05-07 | MSV000087385 | MassIVE
ProteomicsActivity-based SWATH/DIA proteomic screen enables sensitive differential detection of serine hydrolases from surgically resected OCT-embedded primary lung adenocarcinoma
Lung malignancies are among the leading causes of cancer-related death in the modern world. While the disease is relatively manageable if caught at an early stage, the genomic variability and high level of mutations represent a daunting challenge for diagnosis, staging and prognostic classification....
ORGANISM(S): Homo sapiens (Human) 
2025-06-02 | PXD019357 | Pride
Abpp Lung adenocarcinoma tissue Swath-ms 
ProteomicsFirst identification of a large set of serine hydrolases by activity-based protein profiling in dibutyl phthalate-exposed zebrafish larvae
Despite the involvement of several serine hydrolases (SHs) in the metabolism of xenobiotics such as dibutyl phthalate (DBP), no study has focused on mapping this enzyme class in zebrafish, a model organism frequently used in ecotoxicology. Here, we survey and identify active SHs in zebrafish larvae ...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2023-03-11 | PXD038229 | Pride
Proteomics Zebrafish larvae Activity-based protein profiling Serine hydrolases Dibutyl phthalate 
UnknownComputational design of serine hydrolases.
Not available
S-EPMC11384011 | biostudies-literature
ProteomicsSerine Hydrolases from E. Coli
Serine hydrolases from E. Coli cells were labelled with fluorophosphonate probe (ABP-probe): Intact cells labelling by adding probe to media: GPM77700012901 and GPM00300015270 Cell lysate labelling: GPM77700012904 and GPM77700012905 Blank sample (no probe addition): GPM77700012906 and GPM77700012909...
ORGANISM(S): Escherichia coli 
2014-05-20 | PXD000241 | Pride
Activity-based protein profiling E. coli Serine hydrolases Fluorophosphonate probe 
UnknownComputational design of serine hydrolases.
Not available
S-EPMC12288761 | biostudies-literature
ProteomicsThe antimalarial natural product salinipostin A identifies essential alpha/beta serine hydrolases involved in lipid metabolism in P. falciparum parasites
Salinipostin A (Sal A) is a bicyclic phosphotriester natural product isolated from the marine bacterium Salinospora sp. that exhibits potent antimalarial activity. However, the direct targets and mechanisms by which it exerts its effects are poorly understood. Here, we use semi-synthesis to generate...
ORGANISM(S): Plasmodium falciparum 58.1 
2020-01-24 | PXD016835 | Pride
Salinipostin a Serine hydrolase 
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