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ProteomicsBlood plasma and serum samples of healthy donors fractionated on different chromatography sorbents
Blood as connective tissue potentially contain evidence of all processes occurring within the organism, at least in trace amounts. Because of their small size, peptides overcome cell membranes and epithelial barriers more freely than proteins. Among the peptides found in blood, there are both fragme...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-15 | MSV000081717 | MassIVE
ProteomicsMulti-center longitudinal quality assessment of MS-based proteomics in plasma and serum
Advancing MS-based proteomics towards clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, s...
ORGANISM(S): Homo sapiens (Human) 
2025-02-14 | PXD054073 | Pride
Plasma Serum Round robin study R package mpwr Lc-ms/ms 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
TranscriptomicsmiRNA microarray experiment elucidating the contribution of intra-and extracellular leukocyte-derived miRNAs to paired blood serum and plasma samples
This experiment was used to compare the miRNA expression profiles of paired healthy blood serum, blood plasma, blood-derived cellular components and their conditioned media.
ORGANISM(S): Homo sapiens 
2025-06-30 | E-MTAB-14718 | biostudies-arrayexpress
ProteomicsHigh-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry
A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hun...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-08-04 | PXD063043 | Pride
Nanoparticle protein corona Bottom-up proteomics High-throughput Cze-ms/ms Biomarker 
MetabolomicsA Metabonomic Comparison of Human Serum and Plasma Subtypes Using Untargeted NMR Spectroscopy and UPLC-MS (NMR assays)
INTRODUCTION: Differences in the metabolite profiles between serum and plasma are incompletely understood. OBJECTIVES: To evaluate metabolic profile differences between serum and plasma and among plasma sample subtypes. METHODS: We analyzed serum, platelet rich plasma (PRP), platelet poo...
2018-07-25 | MTBLS470 | MetaboLights
ProteomicsA systematic evaluation of the use of human plasma and serum for mass spectrometry-based shotgun proteomics - commercial pooled samples
Over the last two decades, EDTA-plasma has been used as the preferred sample matrix for human blood proteomic profiling. Serum has also been employed widely. Only a few studies have assessed the difference and relevance of the proteome profiles obtained from plasma samples, such as EDTA-plasma or li...
ORGANISM(S): Homo sapiens (Human) 
2018-02-27 | PXD007846 | Pride
Human Ms/ms Serum Plasma Tandem mass tag Li-heparin Edta 
ProteomicsBlood plasma and serum samples of healthy donors fractionated on different chromatography sorbents
Blood as connective tissue potentially contain evidence of all processes occurring within the organism, at least in trace amounts. Because of their small size, peptides overcome cell membranes and epithelial barriers more freely than proteins. Among the peptides found in blood, there are both fragme...
ORGANISM(S): Homo sapiens (Human) 
2017-11-14 | PXD008141 | Pride
Peptidomics Mass-spectrometry Blood plasma and serum Norm Healthy donors 
ProteomicsPlasma Instead of Serum Avoids Critical Confounding of Clinical Metabolomics Studies by Platelets – serum and plasma
Metabolomics is an emerging and powerful bioanalytical method supporting clinical investigations. Serum and plasma are commonly used without rational prioritization. Serum is collected after blood coagulation, a complex biochemical process involving active platelet metabolism. This may affect the me...
ORGANISM(S): Homo sapiens (Human) 
2024-03-25 | PXD041781 | Pride
Confounder Platelets Proteomics Metabolomics Lc-ms Plasma Serum Drug effects 
ProteomicsReanalysis of the blood plasma and serum samples from healthy donors
Previously, we published a dataset of human blood plasma and serum samples of 10 healthy males and 10 healthy females, fractionated on a set of sorbents (cation exchange Toyopearl CM-650M, CM Bio-Gel A, SP Sephadex C-25 and anion exchange QAE Sephadex A-25) and analyzed by LC-MS/MS individually and ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-14 | PXD027587 | Pride
Peptidomics Human microbiota Mass spectrometry Blood plasma and serum Healthy donors 
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