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ProteomicsLong read proteogenomics to characterize protein isoform diversity in human umbilical vein endothelial cells (HUVECs)
Mass spectrometry-based proteomics sample preparation Harvested HUVECs, approximately 5 million cells each, were pelleted and frozen at -80C. The sample pellet was lysed according to the Filter Aided Sample Preparation (FASP) protocol.59 Lysis buffer used in the FASP was changed to 6% SDS, 150 mM DT...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-28 | MSV000089326 | MassIVE
TranscriptomicsUsing RNA-Seq to create sample-specific proteomic databases that enable mass spectrometric discovery of splice junction peptides
Many new alternative splice forms have been detected at the transcript level using next generation sequencing (NGS) methods, especially RNA-Seq, but it is not known how many of these transcripts are being translated. Leveraging the unprecedented capabilities of NGS, we collected RNA-Seq and proteom...
ORGANISM(S): Homo sapiens 
2013-05-17 | E-GEOD-45428 | biostudies-arrayexpress
ProteomicsArterial-Venous Endothelial Cell Cycle Proteins Differential Expression
HUVEC-FUCCI cells were used to demonstrate that different endothelial cell cycle states provide distict windows of opportunity for gene expression in response to extrinsic signals. HUVEC-FUCCI were FACS-isolated into three different cell cycle states. Peptide digests from the resulting lysates showe...
ORGANISM(S): Homo sapiens (Human) 
2022-10-12 | PXD036326 | Pride
Proteomics Proteogenomics Early g1 S/g2/m Endothelial cells Huvec Late g1 Cell cycle 
TranscriptomicsGlobal Identification of Protein PTMs in a Single-pass Database Search
Bottom-up proteomics database search algorithms used for peptide identification cannot comprehensively identify posttranslational modifications (PTMs) in a single-pass because of high false discovery rates (FDRs). A new approach to database searching enables Global PTM (G-PTM) identification by excl...
ORGANISM(S): Mus musculus 
2015-07-31 | E-GEOD-59956 | biostudies-arrayexpress
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