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ProteomicsA Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033065 | Pride
Post-translational modifications (ptms) Phosphopeptides Pancreas N-glycopeptides Dual-functional ti(iv)-immobilized metal affinity chromatography (imac) Electrostatic repulsion hydrophilic interaction chromatography (erlic) Simultaneous enrichment 
ProteomicsTandem Enrichment and Simultaneous Profeling
Tandem Enrichment and Simultaneous Profeling of N-glycopeptides and phosphopeptides
ORGANISM(S): Homo Sapiens 
PXD033282 |
ProteomicsSsimultaneous enrichment of cysteine-containing peptides and phosphopeptides using a novel Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in combination with TiO2 chromatography
Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate most physio-pathological states. Here, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-13 | PXD002704 | Pride
Cyspat  reversible cys modifications  redox chemistry  protein phosphorylation  tio2 enrichment  cross-talk  proteomics 
ProteomicsATP-Coated Dual-functionalized Titanium (IV) IMAC Material for Simultaneous Enrichment and Separation of Glycopeptides and Phosphopeptides
Protein glycosylation and phosphorylation are two of the most common post-translational modifications (PTMs), which plays an important role in many biological processes. However, low abundance and poor ionization efficiency of phosphopeptides and glycopeptides make direct MS analysis challenging. Pr...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-07-20 | PXD029775 | Pride
Phosphoproteomics Lc-ms/ms Glycoproteomics 
GenomicsSimultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series1: no enrichment]
Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series1: no enrichment]
PRJNA764234 | ENA
UnknownSimultaneous enrichment and sequential separation of glycopeptides and phosphopeptides with poly-histidine functionalized microspheres.
Not available
S-EPMC9582455 | biostudies-literature
GenomicsSimultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series2: 6mA DIP enrichment]
Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series2: 6mA DIP enrichment]
PRJNA764233 | ENA
ProteomicsFinding the Sweet Spot in ERLIC Mobile Phase for Simultaneous Enrichment of N-Glyco and Phosphopeptides
Simultaneous enrichment of glyco- and phospho- peptides will benefit the studies of biological processes regulated by these post-translational modifications (PTMs). It will also reveal potential crosstalk between these two ubiquitous PTMs. Unlike custom designed multifunctional solid phase extractio...
ORGANISM(S): Homo sapiens (Human) 
2019-09-06 | PXD015179 | Pride
Mm.1s Glycosylation Erlic Phosphorylation Lc-ms/ms 
UnknownDual-Functional Ti(IV)-IMAC Material Enables Simultaneous Enrichment and Separation of Diverse Glycopeptides and Phosphopeptides.
Not available
S-EPMC8245321 | biostudies-literature
GenomicsDeepH&M: Simultaneous determination of single-CpG hydroxymethylation and methylation levels from enrichment and restriction enzyme sequencing methods
DeepH&M: Simultaneous determination of single-CpG hydroxymethylation and methylation levels from enrichment and restriction enzyme sequencing methods
PRJNA588377 | ENA
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