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ProteomicsLow-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics
Field Asymmetric Ion Mobility Spectrometry (FAIMS) is an indispensable tool in single-cell and low-input proteomics. Here, we show that tuning FAIMS resolution via electrode temperature modulation improves sensitivity. We demonstrate that lowering FAIMS resolution broadens the compensation voltage w...
ORGANISM(S): Homo sapiens (Human) 
2026-04-14 | PXD069335 | Pride
Low-input proteomics Ion mobility filter Single cell proteomics Faims 
ProteomicsFAIMS single cellUltrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell
We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome coverage.
ORGANISM(S): Homo sapiens (Human) 
2020-11-24 | PXD019515 | Pride
Faims Single cell proteomics 
ProteomicsFAIMS single cellUltrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell
We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome coverage.
ORGANISM(S): Homo sapiens (Human) 
2020-12-21 | PXD022791 | Pride
Single-cell proteomics faims 
ProteomicsIon mobility fractionation enhanced mass spectrometry acquisition method for single-cell proteomics
Development and evaluation of the TIFF (Transferring Identification based on FAIMS Fractionation) method by benchmarking with standard methods. Single HeLa cells, LPS-treated RAW cells, and non-depleted human lung dissociated single cells were analyzed as applications of the TIFF method for single c...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2020-08-10 | MSV000085937 | MassIVE
UnknownComprehensive Single-Shot Proteomics with FAIMS on a Hybrid Orbitrap Mass Spectrometer.
Not available
S-EPMC6145172 | biostudies-literature
ProteomicsMultiplexed single cell proteomics of hypoxia in HEK293 expressing PIP-FUCCI
This study aimed to characterize temporal proteome dynamics of hypoxia using single-cell mass spectrometry-based proteomics. HEK293 cells expressing PIP-FUCCI cell cycle markers were grown in suspension culture. Cells were exposed to hypoxia and temporally sampled for single-cell sorting into 384-we...
ORGANISM(S): Homo sapiens (Human) 
2026-02-16 | PXD053053 | Pride
Single cell proteomics Hypoxia Hek293 Scms 
ProteomicsExpanding the depth and sensitivity of cross-link identification by differential ion mobility using FAIMS
In cross-linking mass spectrometry (XL-MS), the depth and sensitivity of cross-link detection is often limited by the low abundance of cross-links compared to non-cross-linked peptides in the digestion mixture. To improve the identification efficiency of cross-links, here we present a gas-phase sepa...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Gallus gallus (Chicken) Equus caballus (Horse) 
2020-07-22 | PXD019926 | Pride
Faims Whole proteome Cross-linking mass spectrometry Xl-ms Orbitrap fusion lumos Cx-ms 
ProteomicsCysteine-directed isobaric labeling combined with GeLC-FAIMS MS for quantitative top-down proteomics
The quantification of proteoforms, i.e., all molecular forms in which proteins can be present, by top-down proteomics provides essential insights into biological processes at the molecular level. Isobaric labeling-based quantification strategies are suitable for multi-dimensional separation strategi...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2025-05-07 | PXD055510 | Pride
Top-down proteomics  tmt labeling  quantitative proteomics  faims  gelc  peppi 
UnknownA fully automated FAIMS-DIA mass spectrometry-based proteomic pipeline.
Not available
S-EPMC10626189 | biostudies-literature
ProteomicsA fully automated FAIMS-DIA mass spectrometry-based proteomic pipeline
Here, we present a standardized, “off-the-shelf” proteomics pipeline working in a single 96-well plate to achieve deep coverage of cellular proteomes with high throughput and scalability. This integrated pipeline streamlining a fully automated sample preparation platform, data independent acquisitio...
ORGANISM(S): Homo sapiens (Human) 
2023-08-22 | PXD029902 | Pride
Dia Ipsc Temporal proteomics Neurodegenerative Single-shot Faims 
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