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RNAs were analysed from a pool of day 7 chicken embryos, the DF-1 chicken fibroblast cell line and chicken bone marrow-derived macrophages (stimulated with CSF-1).
ORGANISM(S): Gallus gallus 
A comparative RNA-SEQ analysis of control total RNA preparations from pooled E14.5 dorsal root ganglia was carried out to determine the performance of the RNAseq reactions at differing concentrations (3ng, 10ng & 30ng in high or low volumes) and concordance among different institutions using the sam...
ORGANISM(S): Mus musculus 
Medium-adapted cultures of H. salinarum were grown in synthetic medium with or without AroAA, and cells harvested by centrifugation after growth had reached OD600nm=0.2 and 0.58, respectively.
ORGANISM(S): Halobacterium salinarum 
The Xenopus mid-blastula transition (MBT) marks the onset of large-scale zygotic transcription, as well as an increase in cell cycle length and a loss of synchronous cell divisions. Little is known about what triggers the activation of transcription or how newly expressed genes interact with each ot...
ORGANISM(S): Xenopus (Silurana) tropicalis 
The primary goal of this experiment is to examine the transcription profiles of HTCC1062 when grown in the presence or absence of D-glucose (30uM final concentration). Experiment confirmed activity of predicted glycolysis operon. Three biological replicates of HTCC1062 grown on glucose (30uM final c...
ORGANISM(S): Candidatus Pelagibacter ubique HTCC1062 
Post-translational modification of proteins has been shown to control different aspects of protein biology. We have previously implicated a SUMO consensus motif in HNF4alpha’s carboxylic terminus as an important regulator of protein biology during stem cell differentiation. In this study, we have ge...
ORGANISM(S): Homo sapiens (Human) 
2019-06-17 | PXD013737 | Pride
In this project we aimed to identify the spectrum of protein binders of nucleolin’s glycine arginine reach (GAR) domain. We did so by designing a 15 amino acid peptide derived from the GAR domain sequence, which includes 3 arginine-glycine-glycine (RGG) repeats and we call "R3". We conducted affinit...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-23 | PXD020804 | Pride
To isolate photoreceptor precursors fit for transplantation, we adapted a 3D differentiation protocol that generates neuroretina from mouse ES cells. We used an adeno-associated viral vector (pseudotype2/9)carrying a GFP reporter under the control of a Rhodopsin promoter (AAV2/9.Rhop.GFP)to select r...
ORGANISM(S): Mus musculus 
Multi-omics single-cell profiling of surface proteins, gene expression and lymphocyte immune receptors from hospitalised COVID-19 patient peripheral blood immune cells and healthy controls donors. Identification of the coordinated immune cell compositional and state changes in response to SARS-CoV-2...
ORGANISM(S): Homo sapiens 
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