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ProteomicsSP3 protocol for proteomic plant sample preparation prior LC-MS/MS
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-26 | PXD022688 | Pride
Protein cleanup Arabidopsis thaliana Bottom-up Mass spectrometry Sds removal Sp3 Fasp Carboxylated magnetic beads 
UnknownDifferences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Cleanup Techniques.
Not available
S-EPMC12333462 | biostudies-literature
ProteomicsBenchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics
Benchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics
ORGANISM(S): Homo sapiens (Human) human gut metagenome 
2024-05-31 | PXD049294 | Pride
Metaproteome Mass spectrometry Fasp Gut microbiota Sample preparation Multiwell plate 
UnknownSolvent Precipitation SP3 (SP4) Enhances Recovery for Proteomics Sample Preparation without Magnetic Beads.
Not available
S-EPMC9330274 | biostudies-literature
ProteomicsAn Integrative SP3 Workflow for Multi-PTM Proteomics Profiling
Global proteomic, redox proteomic, and phosphoproteomic data from mouse C10 epithelial cells and skeletal muscle tissue, no treatments used for method development experiments using these biological sytems. Global proteomic, redox proteomic, phosphoproteomic, and acetylomic data from mouse Beta-TC-6 ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-07-06 | MSV000095264 | MassIVE
ProteomicsDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics
Liquid handling robots have been developed to automate various steps of the bottom-up proteomics workflow, however, protocols for the generation of isobarically labeled peptides remain limited. Existing methods often require costly specialty devices and are constrained by fixed workflows. To address...
ORGANISM(S): Homo sapiens (Human) 
2025-06-16 | PXD060786 | Pride
Automation Sample preperation Unfolded protein response and tandem mass tags 
ProteomicsDIA-MS workflows for cyanobacterial proteomics
In this project we introduce new workflows for the in-depth description of the cyanobacterial proteome. The mainstay features of these workflows are the improvement of sample cleanup and digestion through the use of single-pot solid phase-enhanced sample preparation (SP3), the introduction of a rece...
ORGANISM(S): Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) 
2025-09-15 | PXD062851 | Pride
Dia-ms Cyanobacteria Synechococcus 
UnknownSP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines.
Not available
S-EPMC9010637 | biostudies-literature
UnknownAn Optimized SP3 Sample Processing Workflow for In-Depth and Reproducible Phosphoproteomics.
Not available
S-EPMC12322947 | biostudies-literature
UnknownDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics.
Not available
S-EPMC11888279 | biostudies-literature
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