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TranscriptomicsStallion sperm transcriptome as revealed by microarray analysis and RNA sequencing
Purpose: In order to understand the functional significance of sperm transcriptome in stallion fertility, the aim of this study was to generate a detailed body of knowledge about the sperm RNA profile that defines a normal fertile stallion. Methods: The 50 bp single-end ABI SOLiD raw reads were dire...
ORGANISM(S): Equus caballus 
2013-02-19 | E-GEOD-38725 | biostudies-arrayexpress
ProteomicsBovine sperm surface lectinome
Identification of bovine sperm surface carbohydrate-binding proteins relevant in two important aspects of fertilization: (i) formation of the sperm reservoir in the oviductal epithelium, and (ii) gamete recognition (oocyte-sperm interaction). Using whole sperm cells and a novel affinity capture meth...
ORGANISM(S): Bos taurus (Bovine) 
2016-04-28 | PXD003386 | Pride
Bovine Sperm Lectins Fertilization 
ProteomicsTandem mass spectrometry of buffalo sperm-surface extracted proteins
The buffalo sperm surface proteins which are bound by either non-covalent (electrostatic)interactions or by a GPI-anchor were extracted and subjected to LC-MS/MS. The results revealed that proteins involved in the immune response and reproductive processes adorn the buffalo sperm surface.
ORGANISM(S): Bubalus bubalis bubalis 
2021-08-13 | PXD022114 | Pride
Beta-defensin Sperm Buffalo 
TranscriptomicsThe Partitioning of RNAs in sperm
A diverse pool of RNAs remain encapsulated within the transcriptionally and translationally silent spermatozoon. These transcripts persist within the male gamete despite the dramatic reduction in cellular volume achieved through expulsion of the cytoplasm and quite possibly the nucleoplasm. The prec...
ORGANISM(S): Mus musculus 
2015-07-08 | E-GEOD-62874 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis sperm cells compared to FACS-sorted mature pollen grains and seedlings
Comparative transcriptional profiling of FACS-sorted Arabidopsis thaliana sperm cells versus FACS-sorted mature pollen grains and seedlings
ORGANISM(S): Arabidopsis thaliana 
2008-08-01 | E-ATMX-35 | biostudies-arrayexpress
ProteomicsBovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment
Mammalian fertilization requires a complex interplay between a sperm cell and an oocyte. A significant proportion of these functions are mediated through the proteomic composition of the sperm surface. These proteomic constituents can present as biomarkers to control and regulate breeding of agricul...
ORGANISM(S): Bos taurus (Bovine) 
2015-03-19 | PXD001096 | Pride
Plasma membrane proteome Reproductive biology Biotinylation Bos taurus Sperm cell 
OtherBovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment.
Data from ProteomeXchange, PXD ID: PXD001096. File: 130508_01_Orbi1_SK_SER_G150_T158_BOSS_2xenrich_repl1_try.mzml. Published as part of Proteomics. 2015 Jan 20 . From the Abstract: {{i}} A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm s...
ORGANISM(S): Cow 
GPM32320014064 | GPMDB
Resource Reanalysis 
TranscriptomicsNovel insights into DNA methylation features in spermatozoa: stability and peculiatities
Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether...
ORGANISM(S): Homo sapiens 
2013-07-16 | E-GEOD-47627 | biostudies-arrayexpress
ProteomicsAnalysis of human sperm surface glycoproteins which can interact with galectin-1
Herein, we constructed a human lectin microarray with 60 human lectin and lectin-like proteins. All of the lectins and lectin-like proteins were purified from yeast, and most showed binding to human glycans. To demonstrate the applicability of the human lectin microarray, human sperm were probed on ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003785 | Pride
Nlm Galectin-1 Human sperm 
ProteomicsComprehensive mapping of the bull sperm surface proteome
Nitrogen cavitation of bovine sperm to isolate plasma membrane. FASP digestion using trypsin. Data acquired using 5 workflows: (1) Unfractionated on a 5600 TripleTOF; (2) SCX-ESI-LC-MS/MS on a QSTAR Elite; (3) 1D Gel-LC-ESI-LC-MS/MS on a QSTAR Elite; (4) SCX-LC-MALDI-MS/MS on a UltrafleXtreme; (5) ...
ORGANISM(S): Bos taurus (Bovine) 
2020-01-27 | PXD000007 | Pride
Sperm Ultraflextreme Bovine 5600 tripletof Qstar elite Plasma membrane 
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