In the testis, Serine/arginine protein kinase 1 (SRPK1) and isoform 2 of Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1γ) are essential proteins involved in protamine phosphorylation and dephosphorylation, respectively, and their deregulation is linked to defective chromatin s...
Protein kinases (collectively termed the kinome) represent one of the most tractable drug targets in the pursuit of new and effective cancer treatments. However, less than 20% of the kinome is currently being explored as primary targets for cancer therapy, leaving the majority of the kinome untarget...
To investigate the effect of SRPK1 on gene expression of HEK293T, we used SRPK1 shRNA to stably reduce the expression of SRPK1 via lentiviral transduction, which was then subjected to RNA-seq.
To identify the E3 ubiquitin ligase responsible for the polyubiquitination of SRPK1, we performed immunoprecipitation (IP) using an anti-SRPK1 antibody or IgG control as well as endothelial lysates treated with PA and MG-132, followed by mass spectrometry analysis
To investigate the effect of SRPK1 inhibitor on gene expression of rat corpus cavernosum endothelial cells (RCCECs), we used SRPK1 inhibitor SPHINX31 to treat primary cells, which were then subjected to RNA-seq.
Inactivation of CDK12 characterizes an aggressive sub-group of castration-resistant prostate cancer (CPRC), and CRPC is currently a lethal disease. Hyper-activation of MYC transcription factor is sufficient to confer the CRPC-phenotype. Here, we show that the loss of CDK12 promotes MYC activity, whi...
