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ProteomicsTarget of Dleu2-17aa in CD4+ T cells
To identify Dleu2-17aa micropeptide interacting proteins in CD4+ T cells, we performed pull-down assays fllowed by LC-MS using biotin-labeled Dleu2-17aa. As controls, we included the unlabeled free Dleu2-17aa and a biotin-only group. Pull-downs were carried out in both primary mouse CD4+ T cells and...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-23 | PXD046468 | Pride
Cd4+ t cells Micropeptide 
ProteomicsUbiquitin-based pathway acts inside chloroplasts to regulate photosynthesis
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the acti...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-12-06 | PXD031388 | Pride
Ubiquitinome Chloroplast Organelle Chlorad Proteolysis Ubiquitin 
ProteomicsPP6 interated proteins in HaCaT cells treated with L-menthol
Because HES1 maintained PP6 protein level without affecting its mRNA level, we asked if HES1 directly interacted with PP6 in keratinocytes.
ORGANISM(S): Homo sapiens (Human) 
2022-11-09 | PXD037336 | Pride
Igbp1 Pp6 Hacat L-menthol 
ProteomicsTarget of L-menthol in keratinocytes
To elucidate the PP6-upregulation mechanism of L-menthol, we took advantage of an unbiased target identification approach, DARTS, to identify the target of L-menthol in keratinocytes.
ORGANISM(S): Homo sapiens (Human) 
2022-11-09 | PXD037335 | Pride
Keratinocyte，hes1 L-menthol 
TranscriptomicsGlobal Transcriptional Analysis of Nuclear Reprogramming in the Transition from MEFs to iPSCs
Here, we focused on the intermediate stages of SCR by comparing the somatic cell line induced by OCT4, SOX2, and KLF4 (OSK) for 7 days with mouse embryonic fibroblasts (MEFs), iPSCs, and embryonic stem cells (ESCs). Transcriptional profiles of these four cell lines were analyzed by microarray, and w...
ORGANISM(S): Mus musculus 
2012-10-01 | E-GEOD-37397 | biostudies-arrayexpress
ProteomicsUbiquitin-based pathway acts inside chloroplasts to regulate photosynthesis
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the acti...
ORGANISM(S): Arabidopsis Thaliana 
2022-01-31 | PXD031468 |
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