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TranscriptomicsExtraction of high-quality epidermal RNA after NH4SCN induced dermo-epidermal separation of 4 mm human skin biopsies
To obtain a separation of the epidermal and dermal compartments in order to examine compartment specific biological mechanisms in the skin we incubated 4 mm human skin punch biopsies in ammonium thiocyanate (NH4SCN). We wanted to test 1) the histological quality of the dermo-epidermal separation obt...
ORGANISM(S): Homo sapiens 
2010-03-03 | E-GEOD-15101 | biostudies-arrayexpress
TranscriptomicsTan SingleCell
Transcriptional profiling was performed on 18 cells isolated from libraries produced by culture of cells extracted from neonatal foreskin. The profiling was exploratory in nature, with no pre-defined sample phenotypes except for origin of the cells. Post-hybridization, the single-cell libraries were...
ORGANISM(S): Homo sapiens 
2013-02-18 | E-MTAB-824 | biostudies-arrayexpress
ProteomicsDifferences in Aqueous Humor Protein Profiles in Patients With Proliferative Diabetic Retinopathy Before and After Aflibercept Treatment
To investigate the changes in aqueous humor (AH) protein profiles before and after intravitreal aflibercept (IVA) treatment in patients with proliferative diabetic retinopathy (PDR) patients. 5 PDR patients provided 10 samples of AH before and after IVA treatment (pre-group vs post-group). Proteins ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD039210 | Pride
Proteomics Aflibercept Proliferative diabetic retinopathy Lc-ms/ms 
ProteomicsProteomics Reveals the Potential Mechanism of Tanshinone IIA Promoting the Ex Vivo Expansion of Human Bone Marrow Mesenchymal Stem Cells.
Bone marrow mesenchymal stem cells (BMSCs) is a promising cell type for tissue engineering, however, the application of BMSCs are largely hampered for the limited number harvested from bone marrow cells. The methods or strategies that focused on promoting the capacity of BMSCs expansion ex vivo beco...
ORGANISM(S): Homo sapiens (Human) 
2022-11-28 | PXD036093 | Pride
Bone marrow mesenchymal stem cells  tanshinone iia  proteomics  expansion  fibroblast growth factor 2  autocrine. 
ProteomicsBJ Flag-STING IP Mass Spectrometry
The goal of this project is to identify interacting proteins of Flag-tagged STING before and after cGAMP stimulation. STING knockout (STING-KO) BJ cells stably re-expressing Flag-STING were treated with 1 M cGAMP and whole cell lysates were harvested 30 min later for IP with anti-Flag M2 affinity ge...
ORGANISM(S): Homo sapiens (Human) 
2025-12-07 | PXD059426 | Pride
Immunoprecipitation Sting Cgamp 
ProteomicsTwilight length alters clock function, growth and flowering time in Arabidopsis via LHY/CCA1
In this study we developed an experimental set-up to study variation in twilight length using recently developed 7-band LED lights with fully programmable spectra and sigmoidal ramping of light intensity in controlled growth chambers. We employed this set-up to gain a comprehensive understanding of ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-24 | PXD039428 | Pride
Boxcardia Light signaling Arabidopsis 
TranscriptomicsPhenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis
Background The osmotic regulator OmpR in Escherichia coli regulates differentially the expression of major porin proteins OmpF and OmpC. In Yersinia enterocolitica and Y. pseudotuberculosis, OmpR is required for both virulence and survival within macrophages. However, the phenotypic and regulatory r...
ORGANISM(S): Yersinia pestis 
2011-10-23 | E-GEOD-26601 | biostudies-arrayexpress
ProteomicsUsing L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell.
We used L3MBTL3 knockout MDA-MB-231 cell (L3-KO5+V) and L3MBTL3 rescue MDA-MB-231 cell (L3-KO5+L3). The cells were collected by centrifugation and lysed in lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% NP40, 8% glycerol, protease inhibitor cocktail (#P1010, Beyotime, China) on i...
ORGANISM(S): Homo sapiens (Human) 
2024-10-25 | PXD040150 | Pride
Human Breast cancer cell L3mbtl3 
UnknownEffect of transauricular nerve stimulation on perioperative pain: a single-blind, analyser-masked, randomised controlled trial.
Not available
S-EPMC10080471 | biostudies-literature
ProteomicsOTUD3-Mediated Stabilization of SLC7A11 Drives Sunitinib Resistance by Suppressing Ferroptosis in Clear Cell Renal Cell Carcinoma
After cell lysis, the global proteomes of wild-type 786O and OTUD3-knockdown (KD) 786O cell lines were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Label-free quantification (LFQ) was employed for comparative protein quantification, and tandem mass spectrometry data were...
ORGANISM(S): Homo sapiens (Human) 
2025-08-15 | PXD064004 | Pride
786o shotud3 lc/msms 
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