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Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Being inspired by metabolomic data processing, we have developed a bioinformatic pipeline that optimizes the processing of mass spectral data obtained from isobaric Tandem Mass Tag (TMT) experiments. Our method focuses on the tandem mass spectral level by first quantifying and then identifying (QtI)...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-05-01 | PXD005206 | Pride
Gastroesophageal junction adenocarcinoma (GEJA) is a malignant tumor located at the junction of the esophagus and stomach, and its incidence is increasing yearly, while early screening for biological markers is limited. Tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectromet...
ORGANISM(S): Homo Sapiens 
2021-12-08 | PXD030228 |
Fractionation is essential to achieving deep proteome coverage for sample multiplexing experiments where currently up to 18 samples can be analyzed concurrently. However, prefractionation (i.e., upstream of LC-MS/MS analysis) with a liquid chromatography system constrains sample processing as only a...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD038383 | Pride
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