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ProteomicsTandem MOAC identifies novel in vivo MAP kinase substrates in Arabidopsis thaliana.
Plant Growth and Treatment. Seedlings of Arabidopsis thaliana GVG::FLAG-NtMEK2DD were grown in 50 ml of half-strength Murashige and Skoog medium at 22°C under continuous light (70 µE/m2/sec). Twelve-day-old seedlings were treated with DEX (1 µM) or ethanol (control) and collected 6 h after treatmen...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2012-12-11 | PXD000048 | Pride
Arabidopsis thaliana Ptm Mass spectrometry Map kinases 
UnknownIncremental Modification in the Existing Approaches for Affinity Chromatographic Enrichment of Phosphoproteins Improves Their Profile in Liquid Chromatography-Tandem Mass Spectrometry Analysis.
Not available
S-EPMC11789765 | biostudies-literature
ProteomicsQuantitative Phosphoproteomics Reveals in vivo Boron Deficiency Induced Signaling Dynamics in Arabidopsis Roots
Here we describe an improved tandem MOAC combined phosphoprotein / phosphopeptide enrichment strategy, a scalable phosphoproteomics approach that allows rapid identification of thousands of phophopeptides in plant material. We apply this technology to generate time-resolved maps of boron signaling i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD009836 | Pride
Phosphoproteomics born root 
ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050933 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
UnknownShotgun proteomics of the haloarchaeon Haloferax volcanii.
Not available
S-EPMC2676566 | biostudies-literature
ProteomicsGlobal phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas
Ammonium hydroxide and pyrrolidine eluents were dried (SpeedVac) and reconstituted in 25 ul sample loading buffer (0.1% TFA/2% acetonitrile). Eluent from phosphotyrosine immunoprecipitation was dried and reconstituted in 35 ul of the loading buffer. An LTQ Orbitrap XL (ThermoFisher) in-line with a P...
ORGANISM(S): Homo sapiens (Human) 
2014-01-09 | PXD000658 | Pride
Ms/ms B-cell lymphoma Phosphoproteome 
UnknownAnalysis and prediction of pathways in HeLa cells by integrating biological levels of organization with systems-biology approaches.
Not available
S-EPMC3680226 | biostudies-literature
ProteomicsOptimized NHS-amine labeling for multiplexed phosphoproteome study.
In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate t...
ORGANISM(S): Homo sapiens (Human) 
2018-02-27 | PXD008082 | Pride
Phosphoproteome Β-elimination Hydroxylamine Phosphorylation Tandem mass tag N-hydroxysuccinamide-esters Isobaric tag 
ProteomicsExploration of phosphoproteins in Acinetobacter baumannii
Acinetobacter baumannii is a multidrug-resistant bacterium that has gained significant attention in recent years due to its involvement in a growing number of hospital-acquired infections. The World Health Organization has classified it as a critical priority pathogen, underscoring the urgent need f...
ORGANISM(S): Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2025-09-03 | PXD064268 | Pride
Acinetobacter baumannii  phosphorylation  post-translational modification  proteomic  biofilm  virulence 
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