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ProteomicsHuman MAP4K proteins TAP-LC-MSMS
Using a systematic proteomic approach, we define the protein-protein interaction network for MAP4K family kinases in human cells and reveal diverse cellular signaling events involving this key kinase family. We conducted a proteomic analysis of 7 MAP4K proteins, each with two biological replicates ,...
ORGANISM(S): Homo sapiens (Human) 
2020-06-29 | PXD016931 | Pride
Human Map4k Tap-lc-msms 
ProteomicsHuman WW-domain containing proteins TAP-LC-MSMS
To systematically investigate the regulations and functions of human WW-domain containing proteins, we conducted a proteomic analysis of 26 full-length WW-domain containing proteins and 17 WW-domains only, and identified their associated protein complexes in human HEK293T cells using tandem affinity...
ORGANISM(S): Homo sapiens (Human) 
2019-12-02 | PXD004649 | Pride
Human Lc-msms Ww-domain containing proteins Tap 
ProteomicsHuman Tankyrases TAP-LC-MSMS - Proteomic Analysis of the Human Tankyrase Protein Interaction Network Reveals Its Role in Pexophagy
Tankyrase 1 (TNKS1) and tankyrase 2 (TNKS2) belong to the poly(ADP-ribose) polymerase (PARP) family of proteins, which use NAD+ to modify substrate proteins with ADP-ribose modifications. Tankyrases are implicated involving in multiple cellular functions, including the telomere length control, Wnt/b...
ORGANISM(S): Homo sapiens (Human) 
2017-07-24 | PXD004647 | Pride
Human Lc-msms Tankyrase Tap 
ProteomicsPuf3 Tap Tag and Mass Spectrometry in Not4 Delta and WT Yeast Strains
Investigation of the proteins associated with Puf3, an RNA-binding protein, in Saccharomyces cerevisiae. The goal is to compare the Puf3 interactome in a wild-type strain and in a Not4-deleted strain (Δnot4), in order to assess the potential role of Not4 in modulating Puf3-associated complexes. Puf3...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-09-14 | PXD069853 | Pride
Yeast puf3 tap tag not4 deletion wt ap mass spectrometry yeast 
ProteomicsChaetomium thermophilum Naa50 and Naa15 TMT-labeled LC-MSMS
TMT-labeled LC-MSMS was performed to identify and quantify proteins from three different TAP-purification pull-outs from Chaetomium thermophilum. The aim was to identify interaction partners and to study, whether the two tagged proteins (Naa50 and Naa15) are likely to interact, as they do in other o...
ORGANISM(S): Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 
2022-10-14 | PXD035320 | Pride
Naa50 naa15 lc-msms tmt chaetomium thermophilum 
ProteomicsNumerous post-translational modifications of RNA polymerase II subunit Rpb4 link transcription to post-transcriptional mechanisms
Rpb4/7 binds RNA Polymerase II (Pol II) transcripts co-transcriptionally and accompanies them throughout their lives. By virtue of its capacity to interact with key regulators (e.g., Pol II, eIF3, Pat1) both temporarily and spatially, Rpb4/7 regulates the major stages of the mRNA lifecycle. Here we ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-09-25 | PXD020511 | Pride
Post-translational modifications Rpb4 Mrna 
ProteomicsAmuc_2172 tandem affinity purification LCMSMS
Histone lysine lactylation (Klac) is a new posttranslational modification (PTM) that is installed by acetyltransferase to modulate specific immune responses1 and oncogenesis2,3. Akkermansia muciniphila (A. muciniphila) is a beneficial bacterium that blunts ulcerative colitis (UC) in a mouse model by...
ORGANISM(S): Homo sapiens (Human) 
2023-11-29 | PXD037142 | Pride
Aamuc_2172 tap lc/msms 
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
ProteomicsA comprehensive view on r- protein binding and rRNA domain structuring during early ribosome formation
Formation of the eukaryotic ribosomal subunits follows a strict regime to assemble ribosomal proteins (r-protein) with ribosomal RNAs (rRNA) while removing internal (ITS) and external (ETS) transcribed rRNA spacers. During early stages of large subunit (LSU) formation, ITS2 together with six assembl...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-02-02 | PXD058994 | Pride
Ribosome biogenesis Single particle cryo-em Lsu assembly Ssu processome Rrna domain folding Ribosomal protein assembly 
ProteomicsR2TP_TAP-MS - R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein
The R2TP/Prefoldin-like (R2TP/PFDL) complex is a cochaperone complex involved in the assembly of a number of critical protein complexes. Here we use multiple target tandem affinity purification (TAP) coupled to mass spectrometry to map out the interaction network around R2TP/PFDL.
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006198 | Pride
Human Lc-msms Tap Hek 293 
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