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In Escherichia coli, pH-dependent gene expression varies with oxygen level. Anaerobic pH-dependent expression ratios were analyzed and compared to the published analysis of aerated cultures (Maurer et al, 2005). E. coli K-12 strain W3110 was cultured in closed tubes containing LBK broth buffered a...
ORGANISM(S): Escherichia coli 
Helicobacter pylori causes chronic gastritis and avoids elimination by the immune system of the infected host. The commensal bacterium Lactobacillus acidophilus has been reported to exert beneficial effects as a supplement during H. pylori eradication therapy. In the present study, we applied whole ...
ORGANISM(S): Mus musculus 
The federally endangered Okaloosa darter (Etheostoma okaloosae) is found almost exclusively on the Eglin Air Force Base in the Choctawhatchee Bay watershed of Florida. Portions of this limited habitat are threatened with erosion of soils, altered hydrology, and impaired water quality. One stream rea...
ORGANISM(S): Pimephales promelas 
In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
ORGANISM(S): Xenopus tropicalis 
2024-06-16 | PXD043677 | Pride
In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-06-16 | PXD043676 | Pride
To identify protein phosphatases (PP) that are essential during asexual blood stage development in Plasmodium berghei, we attempted systematic deletion of the 30 P. berghei PP genes using double homologous recombination. Of these, we performed strand-specific RNA-sequencing (RNA-Seq) analysis for 2...
ORGANISM(S): Plasmodium berghei 
In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
ORGANISM(S): Xenopus tropicalis 
2024-06-16 | PXD043680 | Pride
In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-06-16 | PXD043679 | Pride
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