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ProteomicsACBD6-related progressive neurodevelopmental disorder – chemical proteomics of YnMyr-labelled proteins in developing Xenopus tropicalis
In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
ORGANISM(S): Xenopus tropicalis 
2024-06-16 | PXD043677 | Pride
Acbd6 Xenopus tropicalis N-myristoylation Ynmyr 
ProteomicsACBD6-related progressive neurodevelopmental disorder – chemical proteomics of YnMyr-labelled proteins in developing Danio rerio (zebrafish)
In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-06-16 | PXD043676 | Pride
Acbd6 Danio rerio N-myristoylation Ynmyr 
ProteomicsGlobal profiling and inhibition of protein lipidation in vector and host stages of the sleeping sickness parasite Trypanosoma brucei via chemical proteomics
The enzyme N-myristoyltransferase (NMT) catalyses the essential fatty acylation of substrate proteins with myristic acid in eukaryotes and is a validated drug target in the parasite Trypanosoma brucei, the causative agent of African trypanosomiasis (sleeping sickness). N-Myristoylation typically med...
ORGANISM(S): Trypanosoma brucei 
2016-05-17 | PXD004053 | Pride
Target validation N-myristoylation Chemical proteomics Human african trypanosomiasis Click chemistry Protein lipidation 
ProteomicsProfiling N-myristoyltransferase activity and inhibition in breast cancer cells (MD-MB-231) using Sortase-A labelling
We report a novel application for Sortase A (SrtA), labeling N-terminal Gly proteins across the proteome at endogenous levels. We combined SrtA labeling of whole-cell lysates with a potent and selective NMT inhibitor, delivering a method capable of quantifying changes in cellular NMT activity in mul...
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD010634 | Pride
Sortase a  srta  n-myristoylation  nmt 
ProteomicsQuantitative chemical proteomic analysis of YnMyr-tagged proteins in HeLa cells infected with RV16
Rhinovirus causes the common cold and drives exacerbation in asthma and COPD, leading to substantial morbidity and healthcare cost. The single positive strand RNA genome of this non-enveloped virus is translated into a single polyprotein that is processed by virus 3C protease to form three structura...
ORGANISM(S): Homo sapiens (Human) 
2018-05-09 | PXD005798 | Pride
Hela Rv16 Proteomics Myristoylation Nmt inhibition 
ProteomicsMetabolic labeling of NMT substrates by photoactivatable/clickable myristate probes X3/X8/X10
Efficiency of labelling by photoactivatable/clickable myristate probes X3/X8/X10 is compared to the positive control probe YnMyr. Additionally, negative controls for each probe were obtained by treatment with NMT ihibitor IMP-366 (DDD85464). The resulting data indicates efficient labeling of NMT sub...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD027239 | Pride
Nmt Diazirine Metabolic labeling Myristoylation Photocrosslinking 
ProteomicsBi-allelic ACBD6 variants lead to a neurodevelopmental syndrome with progressive complex movement disorders
The acyl-CoA-binding domain-containing protein 6 (ACBD6) is ubiquitously expressed, plays a role in the acylation of lipids and proteins, and regulates the N-myristoylation of proteins via N-myristoyltransferase enzymes (NMTs). However, its precise function in cells is still unclear, as is the conse...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD024957 | Pride
N-myristoylation Metabolic tagging Myristic acid alkyne ynmyr Chemical proteomics Imp-1088 Acbd6 
ProteomicsCo-translational and Post-translational protein myristoylation in HeLa cells
Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. In E. Thinon, R. A. Serwa, et al., Nature Communications 2014, doi:10.1038/ncomms5919, we report the global N-myristoylated prote...
ORGANISM(S): Homo sapiens (Human) 
2018-07-31 | PXD002687 | Pride
Co-translational modification Post-translational modification Apoptosis Protein myristoylation 
ProteomicsGlobal profiling of myristoylation in human lung cancer cells
The experimental design included Calu-1 and H460 cells subjected to four treatment groups (Myr, YnMyr, YnMyr + 10 nM IMP-1088, YnMyr + 100 nM IMP-1088), each with four biological replicates. All samples were ligated to AzDB and affinity enriched. Following digestion, all peptides were subjected to L...
ORGANISM(S): Homo Sapiens 
2026-02-21 | PXD074882 |
ProteomicsValidation and invalidation of chemical probes for the human N-myristoyltransferases.
On-target, cell-active chemical probes are of fundamental importance in both chemical and cell biology, whereas the application of poorly-characterised probes often leads to invalid conclusions. Human N-myristoyltransferase (NMT) has attracted increasing interest as a target in cancer and infectious...
ORGANISM(S): Homo sapiens (Human) 
2019-11-14 | PXD012722 | Pride
N-myristoylation Imp-366 (ddd85646) Metabolic tagging Tris-dba palladium Myristic acid alkyne ynmyr Chemical proteomics Imp-1088 D-nmappd (b13) 2-hydroxymyristic acid 
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