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ProteomicsHuman Sumoylome (SUMO-2) upon bacterial infection
Given the role of SUMOylation in pathogenic infection, we wanted to evaluate the changes to the host SUMO-2 subproteome during Shigella infection. HeLa cells overexpressing TAP-SUMO-2 or TAP-empty were used in conjunction with SILAC (Stable Isotope Labeling of Amino acids in Culture) (Golebiowski ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-19 | PXD001100 | Pride
Human Sumo-2 Shigella flexneri Hela Tap tag 
ProteomicsQuantitative analysis of SUMO1 and SUMO2 proteomes in human lung A549 cells during influenza A virus infection or heat-shock
Dynamic nuclear SUMO modifications play essential roles in orchestrating cellular responses to proteotoxic stress, DNA damageand DNA virus infections. Here, we describe the host SUMOylation response to the nuclear-replicating RNA pathogen, influenz A virus. Using quantitative proteomics to compare S...
ORGANISM(S): Homo sapiens (Human) 
2015-11-05 | PXD002943 | Pride
Influenza Sumo Heat-shock Virus Stress Infection Ubiquitin 
ProteomicsChanges to SUMO-modified proteome and total cellular proteome during EBV ractivation in AGS gastric carcinoma cells
To monitor changes to cellular and viral SUMO-modified proteins during EBV reactivation. SUMO1 and SUMO2 conjugates were studied using KGG mutant forms of SUMO and GG-K specific antibody enrichment of SUMO modified peptides. Time-points of 12h and 24h post-reactivation were monitored. Total cellular...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD034631 | Pride
Sumo epstein-barr virus ebvhuman herpes virus 4 hhv4 
ProteomicsIdentification of SUMO targets required to maintain human stem cells in the pluripotent state. - Identification of SUMO modified proteins from hiPS cells
To determine the role of SUMO modification in the maintenance of pluripotent stem cells we used ML792, a potent and selective inhibitor of SUMO Activating Enzyme. Treatment of human induced pluripotent stem cells with ML792 initiated changes associated with loss of pluripotency such as reduced expre...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD028050 | Pride
Sumo Hipsc Stem cells 
ProteomicsEffect of starvation conditions on cultured human H4 neuroglioma cells stably expressing shLuc and shATG5
An attempt to analyse a large proportion of the human proteome of cultured Human H4 neuroglioma cells during conditions simulating starvation. Comparisons were made under conditions of ATG5 knock-down and control (Luc) knock-down. Whole proteome crude extracts were prepared and comparisons made usin...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD024486 | Pride
Atg5 Cultured human cells Starvation 
ProteomicsMonitoring SUMO-2 proteome changes during adenovirus infection using WT and an E1B-55k mutant.
The human adenovirus type 5 (HAdV-C5) early region 1B 55-kDa protein (E1B-55K) is a multifunctional protein that promotes viral replication and adenovirus-mediated cell transformation through various mechanisms that primarily counteract host intrinsic and innate immunity. These include post-translat...
ORGANISM(S): Homo sapiens (Human) 
2023-10-17 | PXD042236 | Pride
Sumo adenovirus e1b-55k 
UnknownThe pH 6 antigen of Yersinia pestis binds to beta1-linked galactosyl residues in glycosphingolipids.
Not available
S-EPMC108555 | biostudies-literature
ProteomicsAntibody RING-Mediated Destruction (ARMeD) of endogenous proteins
To understand the cellular function of a gene the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the hal...
ORGANISM(S): Homo sapiens (Human) 
2020-05-29 | PXD018113 | Pride
Ring ubiquitin nanobody endogenous degradation 
ProteomicsIdentification of SUMO targets required to maintain human stem cells in the pluripotent state. - Characterisation of 6His-SUMO-KGG-mCherry ChiPS4 cells
To determine the role of SUMO modification in the maintenance of pluripotent stem cells we used ML792, a potent and selective inhibitor of SUMO Activating Enzyme. Treatment of human induced pluripotent stem cells with ML792 initiated changes associated with loss of pluripotency such as reduced expre...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD023241 | Pride
Sumo Stem cells Ml792 
ProteomicsIdentification of SUMO targets required to maintain human stem cells in the pluripotent state. - Total protein level changes in response to ML792 treatment
To determine the role of SUMO modification in the maintenance of pluripotent stem cells we used ML792, a potent and selective inhibitor of SUMO Activating Enzyme. Treatment of human induced pluripotent stem cells with ML792 initiated changes associated with loss of pluripotency such as reduced expre...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD025867 | Pride
Sumo Stem cells Ml792 
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