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To explore the role and target of chloroplast proteases under heat stress, thylakoid membranes were isolated from wild-type and mutant chloroplast thylakoid membrane-localized proteases after heat stress and subjected to comparative quantification by LC-MS/MS analysis using the spectral counting met...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
The thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repai...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-02-11 | PXD046544 | Pride
Plant thylakoid membranes contain hundreds of proteins closely interplaying to cope with ever-changing environmental conditions. We investigated how P. sativum (pea) grown at different irradiances optimizes light-use efficiency through the differential accumulation of thylakoid proteins. Thylakoid m...
ORGANISM(S): Pisum sativum (Garden pea) 
2018-10-02 | PXD009448 | Pride
The plant chloroplast thylakoid membrane must respond to environmental variations in light intensity to maximise the efficiency of photosynthesis and minimise photo-oxidative stress. Plants respond to changing light intensity in the short-term (seconds to minutes) through altered regulation of the s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-08-23 | PXD014982 | Pride
In plants chloroplasts, the lumen of the thylakoid membrane houses proteins that are vital for photosynthetic electron transport, including water-splitting at photosystem (PS) II and shuttling of electrons from cytochrome b6f to PSI. Other lumen proteins maintain photosynthetic activity through biog...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-07-30 | PXD027229 | Pride
A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by ...
ORGANISM(S): Zea mays (Maize) 
2025-10-17 | PXD061472 | Pride
Arabidopsis thaliana Col-0 plants were grown under different light conditions. Chloroplasts were isolated and their proteins extracted. Proteins were analysed by LC-MS and a label free quantification strategy was used to compare the different proteome compositions responsible for the respective accl...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
Quantitative proteome analysis of isolated plasma and thylakoid membrane components.
ORGANISM(S): Synechocystis sp. PCC 6803 
2016-04-12 | PXD003079 | Pride
Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Spinacia sp. 
2026-05-14 | PXD068425 | Pride
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