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ProteomicsMitochondrial CaMKII phosphoproteome.
Mice overexpressing CaMKII in myocardial mitochondria have dilated cardiomyopathy that appears to be exclusively driven by metabolic defects that lead to reduced ATP production. Phosphoproteomics study of mitochondrial proteins supports the metabolomics data.
ORGANISM(S): Mus musculus (Mouse) 
2020-07-31 | PXD004631 | Pride
Heart Tiimac Phosphoproteome Mitochondria Lc-ms/ms 
ProteomicsSpatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for responding and elicit a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spa...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-11-18 | PXD023690 | Pride
Dia Spatio-temporal dynamics Proteomics Phosphoproteomics 
ProteomicsZAKb is a cellular compression sensor that mediates hypertrophic signaling in skeletal muscle
Mechanosensing is required for the senses of touch and hearing, and impacts on cellular processes such as cell differentiation, migration, invasion and tissue homeostasis. Mechanical inputs give rise to p38- and JNK-signaling, which mediates adaptive physiological responses in various tissues. In mu...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-31 | PXD028548 | Pride
Zak Phosphoproteomics Mouse. muscle 
ProteomicsChemogenomic screening in a patient-derived 3D model of metabolic dysfunction-associated steatohepatitis reveals the CHRM1-TRPM8 axis as a novel module for targeted intervention
Metabolic dysfunction-associated steatohepatitis (MASH) is a leading cause of liver disease. A multitude of compounds have been developed in recent years but, due to lack of efficacy or safety concerns, receiving market approval has been challenging and restricted. These failures emphasize the need...
ORGANISM(S): Homo sapiens (Human) 
2024-11-28 | PXD052956 | Pride
Nash  muscarinic receptor  phenotypic assay  target discovery  chemical probes  fibrosis. 
ProteomicsOptimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
In large-scale quantitative mass spectrometry (MS)-based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high-pH reversed-phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affects sensi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-28 | PXD031277 | Pride
Tandem mass tags Phosphoproteomics Optimization High-ph fractionation Isobaric labeling 
ProteomicsMass-spectrometry phosphoproteomics of Calu-3 cell lines infected with SARS-CoV-2, in comparison to non-infected and UV mock-treated cell lines
To identify the proteome alterations in human epithelial cells upon SARS-CoV-2 infection, we performed an in vitro experiment, where we infected lung-adenocarcinoma cultured human airway epithelial cells (Calu-3) with an isolate of the ancestral variant and alpha variant of SARS-CoV-2 in two separat...
ORGANISM(S): Homo sapiens (Human) 
2023-09-01 | PXD040982 | Pride
Covid-19 Phosphosites Ph fractionation Human Sars-cov-2 Calu-3 cell line Infection Phosphoproteomics Lc-ms/ms 
ProteomicsCharacterisation of colorectal cancer cell lines through proteomic profiling of their extracellular vesicles
Colorectal cancer (CRC) is one of the most prevalent cancers, driven by several factors including deregulations in intracellular signalling pathways. Small extracellular vesicles (sEVs) are nanosized protein-packaged particles released from cells, which are present in liquid biopsies. Here, we chara...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD037651 | Pride
Proteomics Sevs Extracellular vesicles Phosphoproteomics Intracellular signalling Colorectal cancer 
ProteomicsGlutamine-fructose-6-phosphate transaminase 2 (GFPT2) is upregulated in breast epithelial-mesenchymal transition and involved in oxidative stress regulation.
Epithelial-mesenchymal transition (EMT) is a diverse and dynamic biological process which is involved in cancer progression. It is important for carcinoma cells during invasion and metastasis. EMT has been in the spotlight for cancer cells to disseminate to distant organs by gaining partial EMT phen...
ORGANISM(S): Homo sapiens (Human) 
2022-01-05 | PXD025858 | Pride
Metabolism Breast cancer Emt Proteomics Oxidative stress Claudin-low Gfpt2 
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