Comprenhensive proteomic analysis of HCEC using Trapped Ion Mobility Spectrometry Time-of-Flight Pro2, Orbitrap Fusion Lumos and ZenoTrap Time-of-Flight 7600 Mass Spectrometer
Glycoproteins play important roles in numerous physiological processes and are often implicated in disease. Analysis of site-specific protein glycobiology through glycoproteomics is evolving rapidly in recent years thanks to hardware and software innovations. Introduction of Parallel Accumulation Se...
Comprenhensive proteomic analysis of HCEC using Trapped Ion Mobility Spectrometry Time-of-Flight Pro2, Orbitrap Fusion Lumos and ZenoTrap Time-of-Flight 7600 Mass Spectrometer
Human ovarian adenocarcinoma OVCAR8 cells and paclitaxel-induced OVCAR8 -Persister cells (3 biological replicates) were collected in lysis buffer and then sonicated three times on ice. The supernatant represented the whole-cell extract. 4D-data independent acquisition (DIA)-based label-free quantita...
This study investigates the toxic effects of the environmental contaminant triclosan (TCS) on porcine oocyte meiosis and the protective role of melatonin (MT). Using an in vitro maturation model, TCS was found to impair polar body extrusion and cumulus expansion, while inducing oxidative stress, mit...
The glymphatic system’s outflow of “dirty” brain fluids collects in the meningeal lymphatic vessels, which drain via lymphatic collectors primarily to the deep cervical lymph nodes (dcLN). It is unclear, however, whether lymphatic dcLN drainage is a prerequisite for normal cerebral homeostasis. Here...
