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ProteomicsHuman pancreatic endocrine cells, LC-MS3
FACS isolated human alpha and beta cells were analyzed by TMT 11-plex labeling, Off-line basic pH reversed phase fractionation and LC-MS3 on an Orbitrap Fusion mass spectrometer.
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD011933 | Pride
Human Endocrine cells Tmt 11-plex Lc-ms3 
ProteomicsEnhanced sample multiplexing-based targeted proteomics with intelligent data acquisition
Targeted proteomics has been playing an increasingly important role in hypothesis-driven protein research and clinical bi-omarker discovery. We have previously created a workflow, Tomahto, to enable real-time targeted pathway proteomics assays using two-dimensional multiplexing technology. Coupled w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-08-14 | MSV000095604 | MassIVE
Proteomicsn Efficient, Amine-Specific, and Cost-Effective Method for TMT 6/11-plex Labeling Improves the Proteome Coverage, Quantitative Accuracy and Precision
Tandem mass tags (TMT) are among the most popular proteomics quantification techniques due to its ability to simulta-neously quantify multiple samples in a single experiment. The tags can be easily added onto the primary amine groups of peptides and proteins through chemical reactions. Although TMT ...
ORGANISM(S): Homo Sapiens 
2023-11-17 | PXD047052 |
ProteomicsSpatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver and Lung Metastasis
Liver, lung and lymph nodes are the most common metatastic sites of colorectal cancer (CRC) cells. Here, we aimed to analyze by quantitative spatial proteomics the isogenic KM12 cell system (non-metastatic KM12C cells, liver metastatic KM12SM cells and liver and lung metastatic KM12L4a cells), and t...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD030671 | Pride
Spatial proteomics Colorectal cancer Tmt 11-plex Cells 
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsEvaluation of differential peptide loading on TMT-based proteomic and phosphoproteomic data quality
Investigating the impacts on MS data quantitation reproducibility when loading differential amounts of peptides, ranging from 20 to 400 ug, across channels of TMT 11 multiplexes. PTRC_Exp9 files represent global proteome and phosphoproteome data generated from PBMCs isolated from AML patients, acqui...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-05 | MSV000086417 | MassIVE
Proteomics27-plex Tandem Mass Tag Mass Spectrometry for Profiling Brain Proteome in Alzheimer's Disease
Systematically optimized the protocol for the newly developed 16-plex TMT Reported a novel 27-plex method to enhance the multiplexity for large-scale proteomic profiling, by combining two sets of distinct isobaric tags (11-plex and 16-plex TMT) based on their disparate mass shifts on modified peptid...
ORGANISM(S): Homo sapiens (Human) 
2021-04-09 | PXD025261 | Pride
Tmt  mass spectrometry  proteomics  proteome  alzheimer’s disease  brain tissue  disease network  aβ 
ProteomicsHEK293 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)
HEK293 cells with two genotype (WT or ATCG5 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex.
ORGANISM(S): Homo sapiens (Human) 
2020-07-12 | PXD017852 | Pride
Human Lc-msms Proteome 
ProteomicsHCT116 cell proteome remodeling upon Torin1 treatment or amino acid withdraw. Related to Supplementary Table 1 from An et al (2020)
HCT116 cells with various genotype (WT, ATCG5 KO, RB1CC1 KO), treated with Torin1 or amino acid withdraw. TMT 11 plex single shot.
ORGANISM(S): Homo sapiens (Human) 
2020-07-13 | PXD017853 | Pride
Human Lc-msms Proteome 
ProteomicsGlobal quantitative analysis of the human brain proteome and phosphoproteome in Alzheimer’s diseaseGlobal quantitative analysis of the human brain proteome and phosphoproteome in Alzheimer’s disease
Alzheimer’s disease (AD) is characterized by an early, asymptomatic phase (AsymAD) in which individuals exhibit amyloid-beta (Aβ) plaque accumulation in the absence of clinically detectable cognitive decline. Here we report an unbiased multiplex quantitative proteomic and phosphoproteomic analysis u...
ORGANISM(S): Homo sapiens (Human) 
2020-09-16 | PXD020296 | Pride
Imac Alzheimer's Tmt Brain Lc ms/ms 
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