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Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
ProteomicsDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics
Liquid handling robots have been developed to automate various steps of the bottom-up proteomics workflow, however, protocols for the generation of isobarically labeled peptides remain limited. Existing methods often require costly specialty devices and are constrained by fixed workflows. To address...
ORGANISM(S): Homo sapiens (Human) 
2025-06-16 | PXD060786 | Pride
Automation Sample preperation Unfolded protein response and tandem mass tags 
UnknownDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics.
Not available
S-EPMC11888279 | biostudies-literature
UnknownDevelopment of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics.
Not available
S-EPMC12150330 | biostudies-literature
ProteomicsProteomics profiling of human brain cortex for JUMPlib manuscript
We generated a large TMT proteomics dataset to evaluate the performance of JUMPlib software (an integrative search tool combining fragment ion indexing with comprehensive TMT spectral libraries). This dataset consists of 40 LC-MS/MS fractions generated by the 16/18-plex TMT strategy from the human b...
ORGANISM(S): Homo sapiens (Human) 
2024-11-04 | PXD057366 | Pride
Mass spectrometry Jumplib software Tmt-based proteomics Human brain cortex 
ProteomicsAssessing Interference in Isobaric Tag-based Sample Multiplexing using an 18-plex Interference Standard
Reporter ion interference remains a limitation of isobaric tag-based sample multiplexing. Advances in instrumentation and data acquisition modes, such as the recently developed real-time database search (RTS), can reduce interference. However, interference persists as does the need to benchmark upst...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-01-06 | PXD029458 | Pride
Faims Eclipse Ifi Sps-ms3 Rts Tmtpro18 
ProteomicsCharacterization of MS white matter derived extracellular vesicles
This dataset contains proteomic profiles of extracellular vesicles (EVs) isolated from human multiple sclerosis (MS) white matter tissue, alongside the corresponding tissue proteome. The aim of this study is to characterize the protein cargo of MS-derived EVs compared to bulk tissue and to identify ...
ORGANISM(S): Homo sapiens (Human) 
2025-11-17 | PXD068695 | Pride
Human brain 
ProteomicsTMT MS RAPIDASH of Mouse macrophages.
Here we are using TMT-based proteomic analysis to characterize changes in the protein content of ribosomal complexes in LPS or poly(I:C) treated vs naive murine macrophages.
ORGANISM(S): Mus musculus (Mouse) 
2025-01-13 | PXD055155 | Pride
Lps Rapidash Affinity purification Tmt Ms Ribosome associated proteins Macrophages Poly(i:c). 
Unknown29-Plex tandem mass tag mass spectrometry enabling accurate quantification by interference correction.
Not available
S-EPMC9588555 | biostudies-literature
ProteomicsTMT-ABPP profiling of SP3CHO in native 293T proteome
This TMT-ABPP experiment was performed in native 293T proteome treated with a range of concentrations of SP3CHO compounds.
ORGANISM(S): Homo sapiens (Human) 
2024-06-24 | PXD051803 | Pride
Abpp Tmt Chemoproteomic 
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