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ProteomicsProfiling Yeast Deletion Strains using Sample Multiplexing and Network-based Analyses
The yeast, Saccharomyces cerevisiae, is a widely used model system for investigating conserved biological functions and pathways. Advancements in sample multiplexing have facilitated the study of the yeast proteome, yet many yeast proteins remain uncharacterized or only partially characterized. The ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-09-02 | PXD032042 | Pride
Deletion strains Yko Rts-ms3 Yeast Tmtpro 
ProteomicsTemporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach
Nicotine is a prominent active compound in tobacco and many smoking cessation products. Some of the biological effects of nicotine are well documented in in vitro and in vivo systems; however, nominal data are available concerning the time-dependent changes on protein and phosphorylation events in r...
ORGANISM(S): Homo sapiens (Human) 
2021-05-11 | PXD023108 | Pride
Faims Tmtpro Covid19 Protein-protein interactions Orbitrap lumos Real time search Rts 
ProteomicsA Semi-automated Paramagnetic Bead-based Platform for Isobaric Tag Sample Preparation
The development of streamlined and high-throughput sample processing workflows is important for capitalizing on emerging advances and innovations in mass spectrometry-based applications. While the adaptation of new technologies and improved methodologies is fast paced, automation of upstream sample ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-01-06 | PXD024282 | Pride
Faims Sp3 Automation Tmtpro Rts Opentrons 
ProteomicsImplementation of Non‑Isobaric TMT Analogues for Accurate Precursor‑Level Quantification by plexDIA
Multiplexed Data-Independent Acquisition (plexDIA) aims to bridge the gap between the quantitative depth of DIA and the sample throughput of isobaric tag-based workflows. Here, we implement non‑isobaric sample multiplexing using commercially available, isotopically differentiated tandem mass tag (TM...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-09-07 | PXD078770 | Pride
Plexdia Tko standard Non-isobaric multiplexing Quantitative proteomics 
ProteomicsDiscovering radiation biomarkers in plasma of total-body irradiated leukemia patients
The population's increasing risk of acute large-scale radiological/nuclear exposures underlines the need for functional radiation biomarkers. As ionizing radiation triggers a complex response on the genome, proteome, and metabolome level, all were already reported as suitable indicators of radiation...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD043516 | Pride
Proteomics Human plasma Irradiation Biomarkers 
ProteomicsTMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics
Isobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here, an expanded set of 16 isobaric reagents based on a proline backbone (TMTpro) is presented. These reagents have similar characteristics to existing TMT reagents but with increased fragmentat...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016491 | Pride
Tmtpro16 
UnknownA Semiautomated Paramagnetic Bead-Based Platform for Isobaric Tag Sample Preparation.
Not available
S-EPMC8210952 | biostudies-literature
UnknownSuper Heavy TMTpro Labeling Reagent: An Alternative and Higher-Charge-State-Amenable Stable-Isotope-Labeled TMTpro Variant.
Not available
S-EPMC8210946 | biostudies-literature
UnknownIsobaric Tag-Based Protein Profiling across Eight Human Cell Lines Using High-Field Asymmetric Ion Mobility Spectrometry and Real-Time Database Searching.
Not available
S-EPMC8106443 | biostudies-literature
UnknownProfiling Yeast Deletion Strains Using Sample Multiplexing and Network-Based Analyses.
Not available
S-EPMC9614305 | biostudies-literature
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