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MetabolomicsLCMS analysis of seven apple varieties with a leaking chromatographic column
The experiment contains data from a metabolomics experiment on different apple varieties. Quality Control and Standard Mixture samples are also included. In this study, retention times are extremely variable because of a problem with the chromatographic column.
2015-06-05 | MTBLS99 | MetaboLights
ProteomicsMitigating in-column artificial modification in HTLC for bottom-up proteomics and quality control of biopharmaceuticals
The study aimed to investigate the effect of an additional short trap column installed upstream of the separation column on the in-column artificial modification and separation performance in high-temperature LC-MS analyses of peptides.
ORGANISM(S): Francisella tularensis subsp. holarctica (strain LVS) Homo sapiens (Human) 
2024-09-11 | PXD052716 | Pride
Biopharmaceuticals High-temperature liquid chromatography Column temperature Trap column Bottom-up proteomics Artifacts 
ProteomicsTrapping efficiency of peptides on particle-packed and micro-pillar trap columns for proteomics analyses
In current work, we compared the separation of HeLa samples using two different trap columns for sample trapping, desalting, and preconcentration. The two columns have different types of the C18 stationary phase: a) a conventional packed PepMap C18 trap-column (Thermo Fischer Scientific), and b) the...
ORGANISM(S): Homo sapiens (Human) 
2026-02-18 | PXD052759 | Pride
Peptides Proteomics Nano Micropillar Trap column 
UnknownTrap column-based intact mass spectrometry for rapid and accurate evaluation of protein molecular weight.
Not available
S-EPMC9126647 | biostudies-literature
TranscriptomicsTranscription profiling of Arabidopsis plants with the chlorophyll removed by combining enhancer trap and RNAi approaches
Chlorophyll was removed from the veins of the C3 plant Arabidopsis by combining enhancer trap and RNAi approaches. Three independent transgenic lines were created. These were each compared to the parent line, J1511, in a microarray experiment.
ORGANISM(S): Arabidopsis thaliana 
2009-12-01 | E-MEXP-1988 | biostudies-arrayexpress
UnknownParallelization with Dual-Trap Single-Column Configuration Maximizes Throughput of Proteomic Analysis.
Not available
S-EPMC9900495 | biostudies-literature
ProteomicsOpen-Tubular Trap Columns: Towards Simple and Robust Liquid Chromatography Separations for Single-Cell Proteomics
Nanoflow liquid chromatography-mass spectrometry is key to enabling in-depth proteome profiling of trace samples such as single cells, but these separations can lack robustness due to the use of narrow-bore columns that are susceptible to clogging. In the case of single-cell proteomics, offline clea...
ORGANISM(S): Homo sapiens (Human) 
2024-02-09 | PXD048565 | Pride
Solid-phase extraction Trap column Open-tubular Single-cell proteomics 
UnknownHigh-Throughput Proteomics Enabled by a Fully Automated Dual-Trap and Dual-Column LC-MS.
Not available
S-EPMC12752021 | biostudies-literature
ProteomicsComplement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument
Proteomics workflows have traditionally been divided into discovery-based and targeted approaches, with instrumentation optimized for each. Discovery experiments typically utilize high-resolution analyzers such as the Orbitrap™, while targeted workflows rely on the sensitivity and specificity of tri...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2025-09-08 | PXD062540 | Pride
Stellar Complement reporter ion Quadrupole-ion trap Multiplexed shotgun proteomics 
ProteomicsSubstrates of human TRAP complex
While the Sec61-complex in the membrane of the human endoplasmic reticulum facilitates translocation of all precursor polypeptides with amino-terminal signal peptides or transmembrane helices, the Sec61-associated translocon-associated protein (TRAP)-complex supports translocation of only a subset o...
ORGANISM(S): Homo sapiens (Human) 
2018-08-09 | PXD008178 | Pride
Sec61 complex Ms Hela cells Er protein import Trap complex 
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