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Proteomicsubiquitin chain linkage of uS10
Quantification of ubiquitin chain linkage of uS10. Quantification of ubiquitin chain linkage of uS10in UbiR54A mutant strain.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-09-18 | PXD052346 | Pride
Ubiquitin chain linkage Us10 Yeast 
ProteomicsProteomic Analysis of Ubiquitin Chain Types in Murine Cells and Tissues
Mass spectrometry-based proteomics has become an integral approach for characterising ubiquitin chain-linkage compositions and architectures. In this study, we optimised sample preparation and chromatographic separation of Ubiquitin peptides for Absolute Quantification by Parallel Reaction Monitorin...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-28 | PXD017754 | Pride
Absolute quantification Ubiquitin chain-linkage composition Murine tissues Parallel reaction monitoring 
ProteomicsLinkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler
Virtually all aspects of cell biology are regulated by a ubiquitin code where distinct ubiquitin chain architectures guide the binding events and itineraries of modified substrates. Various combinations of E2 and E3 enzymes accomplish chain formation by forging isopeptide bonds between the C-terminu...
ORGANISM(S): Homo sapiens (Human) 
2020-09-09 | PXD021286 | Pride
Proteomics Ubiquitin 
ProteomicsUbiquitin diGly site ampping on ZNFX1 autoubiquitination reactions
This experiment aimed to characterise ubiquitin chain linkage preferences generated and autoubiquitination sistes during ZNFX1 E3 reactions using diGly remnant mass spectrometry. GlyGly-modified lysine residues were identified as a qualitative proxy for ubiquitin linkage types. Spectral counts of ra...
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD073484 | Pride
Znfx1 ubiquitin chains 
ProteomicsLinkage Reprogramming by Tailor-made E3s Reveals Polyubiquitin Chain Requirements in DNA Damage Bypass
A polyubiquitin chain can adopt a variety of shapes, depending on how the ubiquitin monomers are joined. However, the relevance of linkage for the signaling functions of polyubiquitin chains is often poorly understood because of our inability to control or manipulate this parameter in vivo. Here we ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-04-25 | PXD030735 | Pride
Ubiquitin E3 ligase Dna damage bypass Pcna 
ProteomicsProfiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome maintenance
Linkage-specific ubiquitin (Ub) chains dictate the functional outcome of many critical Ub-dependent signaling processes. However, the functions and targets of several poly-Ub topologies remain poorly understood due to a lack of tools for their specific detection and manipulation. To remedy this know...
ORGANISM(S): Homo sapiens (Human) 
2025-09-18 | PXD056493 | Pride
Ubiquitin  ubiquitin-proteasome system  k29-linked ubiquitylation  chromatin  epigenome 
GenomicsProfiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome integrity
Profiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome integrity
PRJNA1171407 | ENA
ProteomicsA novel polyubiquitin chain linkage formed by viral Ubiquitin resists cleavage by deubiquitinating enzymes
The project reports the structural difference between viral Ubiquitin (vUB) and human ubiquitin, hence causing local and global destabilization in vUB. Viral Ubiquitin conjugates are degraded by proteasome machinery. The in-vitro biochemical assays suggest comparable activity of mono UB and mono-vUB...
ORGANISM(S): Bombyx mori (Silk moth) 
2020-06-28 | PXD017215 | Pride
Ubiquitination Dubs Nmr Lc-ms Isopeptide bond Proteasomal degradation 
ProteomicsUbiquitin S65 proteomics: Targets of ubiquitin phosphorylation
Ubiquitylation is an essential post-translational modification that regulates numerous cellular processes, most notably protein degradation. Ubiquitin itself can be post-translationally modified by phosphorylation, with nearly every serine, threonine, and tyrosine residue having the potential to be...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-07-07 | PXD002201 | Pride
Yeast Orbitrap Phosphorylation Ubiquitin 
ProteomicsUbiquitin S65 proteomics: Association of ubiquitin mutants with ubiqiutin binding domains
Ubiquitylation is an essential post-translational modification that regulates numerous cellular processes, most notably protein degradation. Ubiquitin itself can be post-translationally modified by phosphorylation, with nearly every serine, threonine, and tyrosine residue having the potential to be...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-07-07 | PXD002202 | Pride
Yeast Orbitrap Phosphorylation Ubiquitin 
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