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ProteomicsGlycan-induced structural dynamics in human norovirus P dimers depend on the virus strain and deamidation status
Noroviruses are non-enveloped (+) single stranded RNA viruses of the Caliciviridae family that cause an estimated 20 % of gastroenteritis cases worldwide . The virus possesses an icosahedral capsid, build by the two structural proteins VP1 and VP2. Noroviruses of genogroup II, especially GII.4, domi...
ORGANISM(S): Norwalk virus Norovirus Hu/GII.4/Saga4/2009/JP Norovirus Hu/GII/JP/2015/GII.P17_GII.17/Kawasaki308 Norovirus Hu/GII.4/MI001/2011/USA 
2021-04-08 | PXD019884 | Pride
Protein dynamics Norovirus Glycan Hdx-ms Interaction Deamidation 
ProteomicsMapping the peptide binding groove of MHC class I
An essential element of adaptive immunity is the selective binding of peptide antigens by major histocompatibility complex (MHC) class I proteins and their presentation to cytotoxic T lymphocytes on the cell surface. Using native mass spectrometry, we here analyze the binding of peptides to an empty...
ORGANISM(S): Homo sapiens (Human) 
2022-05-31 | PXD027725 | Pride
Peptide-binding Hla-a Native ms Human Mhc class i 
ProteomicsDissociation of β2m from MHC Class I triggers formation of noncovalent, transient heavy chain dimers
At the plasma membrane of mammalian cells, major histocompatibility complex class I molecules (MHC-I) present antigenic peptides to cytotoxic T cells. Following the loss of the peptide and the light chain beta-2 microglobulin (beta2m), the resulting free heavy chains (FHCs) can associate into homoty...
ORGANISM(S): Homo sapiens (Human) 
2022-05-11 | PXD033485 | Pride
Hla-a Dimerization Native ms Human Mhc class i 
ProteomicsInteractions of human norovirus capsid P domain with histo-blood group antigens with Hydrogen-Deuterium Exchange Mass Spectrometry
Attachment of human noroviruses to histo blood group antigens (HBGAs) is essential for infection, but how this binding event promotes the infection of host cells is unknown. Here, we employed protein NMR experiments supported by mass spectrometry and crystallography to study HBGA binding to the P ...
ORGANISM(S): Norwalk virus Norovirus Hu/GII/JP/2015/GII.P17_GII.17/Kawasaki308 Norovirus Hu/GII.4/MI001/2011/USA Norovirus Hu/GII-4/Saga4/2006/JP 
2019-03-20 | PXD011914 | Pride
Hdx ms P domain Gii.4 saga Human norovirus Histo-blood group antigens 
ProteomicsNative mass spectrometry of peptide-free and partially loaded MHC class I molecules
A disulfide-stabilized MHC class I molecule (HLA-A*02:01(Y84C/A139C), short dsA2) was characterized by native mass spectrometry. dsA2 was shown to be structurally intact in absence of any peptide whereas its wild type form was not. Furthermore, it was observed that the mutation did not disrupt the b...
ORGANISM(S): Homo sapiens (Human) 
2022-05-11 | PXD016708 | Pride
Peptide-binding Hla-a Native ms Human Mhc class i 
ProteomicsNative mass spectrometry of calmodulin binding to the plasma-membrane Ca2+-ATPase ACA8
Binding of two calmodoulin proteins to the integral membrane protein ACA8, a plasma-membrane Ca2+-ATPase von A. thaliana, was shown by native mass spectrometry.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-12-05 | PXD011177 | Pride
Arabidopsis thaliana Native ms Membrane protein 
ProteomicsStatistical crystallography reveals an allosteric network in SARS-CoV-2 Mpro
To interpret and transmit biological signals, proteins use correlated motions. Experimental determination of these dynamics and the structural distributions they generate remains a key challenge. Here, using 1146 crystal structures of the main protease (Mpro) from SARS-CoV-2, we were able to infer a...
ORGANISM(S): Escherichia coli 
2026-04-14 | PXD076496 | Pride
Enzymology Sars-cov-2 Crystallography 
ProteomicsN-terminal VP1 truncation favor T=1 norovirus-like particles
Noroviruses cause immense sporadic gastroenteritis outbreaks worldwide. Upcoming genotypes, which are divided based on VP1 sequence, further enhance this public thread regularly. Self-assembling properties of the human norovirus major capsid protein VP1 are crucial for using virus-like particles (VL...
ORGANISM(S): Norovirus 
2021-01-11 | PXD023182 | Pride
Capsid assembly Norovirus Vp1 truncation 
ProteomicsRNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment
Lassa virus is a negative-strand RNA virus with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming the ribonucleoparticles together with the viral RNA and the L protein, which have to be continuously restructured...
ORGANISM(S): Lassa mammarenavirus 
2023-12-18 | PXD040186 | Pride
Native ms Lassa virus 
ProteomicsThe kinetics of nsp7-11 polyprotein processing and impact on complexation with nsp16 among human coronaviruses
In coronavirus (CoV) infection, polyproteins (pp1a/pp1ab) are processed into non-structural proteins (nsps), which largely form the replication/transcription complex (RTC). The polyprotein processing and complex formation is critical and offers potential therapeutic targets. However, the interplay o...
ORGANISM(S): Escherichia coli 
2025-07-08 | PXD049009 | Pride
Mass spectrometry Sars-cov-2 Kinetics Native ms Rate constants Complexation 
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