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ProteomicsUltrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
Extracellular vesicles (EVs) have a great potential in clinical applications, however their isolation from different body fluids and their characterisation are not yet optimal or standardised. Here we report the results of examining the performance of ultrafiltration combined with size exclusion chr...
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD009709 | Pride
Urine Extracellular vesicle Exosome Orbitrap Lc-ms/ms 
ProteomicsUltrafiltration combined with Size Exclusion Chromatography efficiently isolates Extracellular Vesicles from Cell Culture Media for compositional and functional Studies
Extracellular vesicles (EVs) exert important functions in the extracellular space and in cell-to-cell communication. Ultracentrifugation, the most frequently used EV isolation technique, requires specialized equipment and provides relatively low purity and yield. A more recently applied and easily a...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006738 | Pride
Extracellular vesicles Lc-msms 
UnknownUltrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine.
Not available
S-EPMC6032024 | biostudies-literature
UnknownSize-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease.
Not available
S-EPMC9963337 | biostudies-literature
UnknownUltrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
Not available
S-EPMC5681555 | biostudies-literature
ProteomicsTowards optimised extracellular vesicle proteomics from cerebrospinal fluid
The proteomic profile of extracellular vesicles (EVs) from cerebrospinal fluid (CSF) can reveal novel biomarkers for diseases of the brain. Here, we validate an ultrafiltration combined with size-exclusion chromatography (UF-SEC) method for isolation of EVs from canine CSF and probed the effect of s...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2023-07-20 | PXD036748 | Pride
Proteomics Ultrafiltration combined with size-exclusion chromatography Extracellular vesicles Cerebrospinal fluid 
ProteomicsA comparison of blood plasma exosome enrichment strategies for proteomic analysis.
Proteomic analysis of exosomes (EXs) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Methods were evaluated for their capacity to successfully isolate and enrich EXs from plasma, minimise the presence of hig...
ORGANISM(S): Bos taurus (Bovine) 
2022-08-12 | PXD032313 | Pride
Exosome  isolation  enrichment  ultracentrifugation  size-exclusion chromatography  ultrafiltration  mass-spectrometry  proteomics  extracellular vesicles 
UnknownSize-Exclusion Chromatography Separation Reveals That Vesicular and Non-Vesicular Small RNA Profiles Differ in Cell Free Urine.
Not available
S-EPMC8124894 | biostudies-literature
ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, Wheat Germ.
The associated files are mass spec data from Size exclusion or Ion exchange chromatography fractions of soluble native wheat germ extract (Triticum aestivum).
ORGANISM(S): Triticum aestivum (Wheat) 
2019-10-28 | PXD013300 | Pride
Triticum aestivum Ion exchange Size exclusion Wheat germ Cf-ms 
UnknownSize-Exclusion Chromatography: A Path to Higher Yield and Reproducibility Compared to Sucrose Cushion Ultracentrifugation for Extracellular Vesicle Isolation in Multiple Myeloma.
Not available
S-EPMC11313515 | biostudies-literature
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