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ProteomicsEpi-proteomic assessment of backpain therapy
This study investigates how molecular profiles evolve in response to standardized sport therapy and non-controlled lifestyle choices. We report that the therapy improves agility, attenuates backpain, and also triggers healthier habits. We find that a subset of participants´ histone methylation and a...
ORGANISM(S): Homo sapiens (Human) 
2024-10-21 | PXD050113 | Pride
Pbmc Histone ptms Buccal swabs Proteome Backpain therapy 
ProteomicsRIF1 orchestrates a multi-step restoration of post-replicative H3K9me3
DNA replication causes the dilution of parental histones along with their specific post-translational modifications. The kinetics of restoring these marks on newly incorporated histones dictate how quickly genomic domains regain their epigenetic identity after replication. H3K9me3 is restored extrem...
ORGANISM(S): Homo sapiens (Human) 
2026-05-18 | PXD066172 | Pride
Human Replication Histones 
ProteomicsIn vitro chromatin assembly - A quantitative proteomic analysis of in vitro assembled chromatin
Here, we use LC-MSMS and SWATH-MS to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos. This system allows easy manipulation of distinct aspects of chromatin assembly such as post-translational histone mod...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-01-27 | PXD003445 | Pride
Lc-msms Chromatin assembly Drosophila melanogaster 
ProteomicsDomain model explains propagation dynamics and stability of K27 and K36 methylation landscape
Chromatin states must be stably maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications across cell division is thought to be central in this process. However, the histone modification landscape is challenged by the incorporation of...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-29 | PXD014807 | Pride
Q-exactive hf Chromatin domains Histone modifications Data modeling 
ProteomicsProfiling linkage-specific ubiquitin chain functions reveals a key role of K29-linked ubiquitylation in epigenome maintenance
We applied a cell-based Ub replacement strategy enabling specific, conditional abrogation of each of the seven lysine-based Ub chain types in human cells to comprehensively profile global ubiquitylation changes resulting from disabling formation of individual Ub chain types. Focusing on K29-linked u...
ORGANISM(S): Homo sapiens (Human) 
2025-09-18 | PXD056295 | Pride
Human Ubiquitylation Ptm 
ProteomicsHistone modifications during acute S-phase defects
Long-term perturbation of de novo chromatin assembly during DNA replication has profound effects on epigenome maintenance and cell fate. The early mechanistic origin of these functional defects is unknown. Here, we combine acute degradation of Chromatin Assembly Factor 1 (CAF-1), a key player in de ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-14 | PXD050618 | Pride
Cell cycle Chromatin assembly Histone chaperones Epigenome stability Dna replication 
ProteomicsHistone dynamics at replication forks in embryonic stem cells
We employ inducible mutants to dissect the early and late consequences of impaired histone recycling. We find rapid accumulation of H3K27me3 in response to impaired histone recycling, preceding gene expression changes. Simultaneous disruption of both leading (POLE4) and lagging strand (MCM2-2A) recy...
ORGANISM(S): Mus musculus (Mouse) 
2025-01-12 | PXD053300 | Pride
Parental histones Pole4 Mcm2 Escs 
ProteomicsIdentification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry
Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified PWWP2A, which tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro a...
ORGANISM(S): Homo sapiens (Human) 
2018-08-27 | PXD010424 | Pride
Pwwp2a / h2a.z / h3k36me3 / chromatin / nurd / mta1 
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