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ProteomicsHeLa_cell_Human_CLN5-overexpression_IP-MS
Overexpressed CLN5 were tagged by FLAG and then purified by TAP with FLAG M2 beads. Then the product is sbujected to MS to ananlyze the interaction proteins with CLN5.
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD028480 | Pride
Hela Cln5-ove Ip-ms 
ProteomicsHuman_HeLa endogenous KCTD7_IP-MS
endogenous KCTD7 were tagged by FLAG and then purified by TAP with FLAG M2 beads. The then sbujected to MS to ananlyze the interaction proteins with KCTD7.
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD028479 | Pride
Human Kctd7 Hela Ip-ms 
ProteomicsThe identification of phosphorylation sites of IBTK by shotgun proteomics
Translation is a tightly regulated process, and the mTORC1-S6K signaling axis plays a critical role in this control. Binding of eIF4F to the cap is hindered by eIF4E binding proteins (4EBPs), which, when hypophosphorylated, sequester eIF4E and prevent its association with eIF4G. However, in response...
ORGANISM(S): Homo sapiens (Human) 
2024-05-10 | PXD038307 | Pride
Ibtk Phosphorylation Shotgun 
ProteomicsThe identification of ubiquitination sites of IBTK by shotgun proteomics
Previous study reported that eIF4A1 protein stability is subject to control by the ubiquitin-proteasome pathway, and deubiquitinase USP9X elevated eIF4A1 protein level by promoting eIF4A1 deubiquitination. However, the E3 ubiquitin ligases responsible for eIF4A1 proteolysis is not determined. Our re...
ORGANISM(S): Homo sapiens (Human) 
2024-05-10 | PXD039031 | Pride
Ibtk Ubiquitination Shotgun 
ProteomicsDefine the interactome of IBTK by Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods
Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods are powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex, BioID labe...
ORGANISM(S): Homo sapiens (Human) 
2024-05-10 | PXD038286 | Pride
Ap-ms Ibtk interactome Bioid 
ProteomicsIdentify the interacting proteins of FBXL4 by AP-MS
Affinity purification coupled with mass spectrometry (AP-MS) is powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex.Mass spectrometry was performed to identify the interacting proteins of FBXL4 fro...
ORGANISM(S): Homo sapiens (Human) 
2024-05-12 | PXD040601 | Pride
Human Fbxl4 Ap-ms 
ProteomicsSPOP mass spec in different treatment
293T cells were transfected with S, Flag, and Biotin-binding-protein-(streptavidin)-binding-peptide (SFB) triple-tagged backbone vector or SFB-tagged SPOP. Twenty-four h after transfection, control cells were treated with DMSO (Group 1) and SFB-SPOP transfected cells were treated with DMSO (Group 2)...
ORGANISM(S): Homo Sapiens 
2021-09-06 | PXD028210 |
ProteomicsSPOP mutation induces replication over-firing by impairing Geminin ubiquitination and triggers replication catastrophe upon ATR inhibition
Geminin ubiquitin modification site mapping, Geminin coIP-mass spec
ORGANISM(S): Homo Sapiens 
2021-08-12 | PXD027915 |
ProteomicsRNF25-Mediated NF-κB Activation Drives Anti-Apoptosis and Therapy Resistance in Cancer
Dysregulation of the NF-κB pathway is frequently observed in cancers, where it contributes to therapy resistance by inhibiting apoptosis. Mass spectrometry (MS)-based proteomics were employed to identify factors associated with the anti-apoptotic process. We identify RNF25, an E3 ubiquitin ligase, a...
ORGANISM(S): Homo Sapiens 
2025-03-05 | PXD061521 |
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